Browsing by author "Willison, Keith"
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Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins
Goyder, MS; Willison, KR; Klug, DR; deMello, AJ; Ces, O (KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2012-04-30)We present a top down separation platform for yeast ribosomal proteins using affinity chromatography and capillary electrophoresis which is designed to allow deposition of proteins onto a substrate. FLAG tagged ribosomes ... -
Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA
Noad, RJ; Stewart, M; Boyce, M; Celma, CC; Willison, KR; et al. (BIOMED CENTRAL LTD, 2009-09-02)Background: Many cellular multi-protein complexes are naturally present in cells at low abundance. Baculovirus expression offers one approach to produce milligram quantities of correctly folded and processed eukaryotic ... -
Protein identification and quantification by two-dimensional infrared spectroscopy: Implications for an all-optical proteomic platform
Fournier, F; Gardner, EM; Kedra, DA; Donaldson, PM; Guo, R; et al. (NATL ACAD SCIENCES, 2008-10-07)Electron-vibration-vibration two-dimensional coherent spectroscopy, a variant of 2DIR, is shown to be a useful tool to differentiate a set of 10 proteins based on their amino acid content. Two-dimensional vibrational ...