Browsing ICR Divisions by author "Coster, Gideon"
Now showing items 1-5 of 5
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Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading.
Coster, G; Diffley, JFX (AMER ASSOC ADVANCEMENT SCIENCE, 2017-07-21)Bidirectional replication from eukaryotic DNA replication origins requires the loading of two ring-shaped minichromosome maintenance (MCM) helicases around DNA in opposite orientations. MCM loading is orchestrated by binding ... -
Cytosine base modifications regulate DNA duplex stability and metabolism.
Rausch, C; Zhang, P; Casas-Delucchi, CS; Daiß, JL; Engel, C; et al. (OXFORD UNIV PRESS, 2021-12-16)DNA base modifications diversify the genome and are essential players in development. Yet, their influence on DNA physical properties and the ensuing effects on genome metabolism are poorly understood. Here, we focus on ... -
Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase.
Coster, G; Frigola, J; Beuron, F; Morris, EP; Diffley, JFX (CELL PRESS, 2014-09-04)Loading of the six related Minichromosome Maintenance (MCM) proteins as head-to-head double hexamers during DNA replication origin licensing is crucial for ensuring once-per-cell-cycle DNA replication in eukaryotic cells. ... -
Replication-induced DNA secondary structures drive fork uncoupling and breakage.
Williams, SL; Casas-Delucchi, CS; Raguseo, F; Guneri, D; Li, Y; et al. (WILEY, 2023-11-15)Sequences that form DNA secondary structures, such as G-quadruplexes (G4s) and intercalated-Motifs (iMs), are abundant in the human genome and play various physiological roles. However, they can also interfere with replication ... -
The mechanism of replication stalling and recovery within repetitive DNA.
Casas-Delucchi, CS; Daza-Martin, M; Williams, SL; Coster, G (NATURE PORTFOLIO, 2022-07-19)Accurate chromosomal DNA replication is essential to maintain genomic stability. Genetic evidence suggests that certain repetitive sequences impair replication, yet the underlying mechanism is poorly defined. Replication ...