Now showing items 1-9 of 9

    • Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement. 

      Patel, AB; Moore, CM; Greber, BJ; Luo, J; Zukin, SA; Ranish, J; Nogales, E (2019-12-30)
      Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a ...
    • Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. 

      Bock, LJ; Pagliuca, C; Kobayashi, N; Grove, RA; Oku, Y; Shrestha, K; Alfieri, C; Golfieri, C; Oldani, A; Dal Maschio, M; Bermejo, R; Hazbun, TR; Tanaka, TU; De Wulf, P (2012-05-06)
      Kinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1(Spc105), ...
    • An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules. 

      Bakos, G; Yu, L; Gak, IA; Roumeliotis, TI; Liakopoulos, D; Choudhary, JS; Mansfeld, J (2018-11-14)
      Covalent modifications of proteins with ubiquitin and ubiquitin-like molecules are instrumental to many biological processes. However, identifying the E3 ligase responsible for these modifications remains a major bottleneck ...
    • Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation. 

      Greber, BJ; Gerhardy, S; Leitner, A; Leibundgut, M; Salem, M; Boehringer, D; Leulliot, N; Aebersold, R; Panse, VG; Ban, N (2016-01)
      Eukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, which includes the ...
    • Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. 

      Gouge, J; Satia, K; Guthertz, N; Widya, M; Thompson, AJ; Cousin, P; Dergai, O; Hernandez, N; Vannini, A (2015-12)
      TFIIB-related factor 2 (Brf2) is a member of the family of TFIIB-like core transcription factors. Brf2 recruits RNA polymerase (Pol) III to type III gene-external promoters, including the U6 spliceosomal RNA and selenocysteine ...
    • RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex. 

      Martino, F; Pal, M; Muñoz-Hernández, H; Rodríguez, CF; Núñez-Ramírez, R; Gil-Carton, D; Degliesposti, G; Skehel, JM; Roe, SM; Prodromou, C; Pearl, LH; Llorca, O (2018-04-16)
      The R2TP/Prefoldin-like co-chaperone, in concert with HSP90, facilitates assembly and cellular stability of RNA polymerase II, and complexes of PI3-kinase-like kinases such as mTOR. However, the mechanism by which this ...
    • Structural basis of RNA polymerase III transcription initiation. 

      Abascal-Palacios, G; Ramsay, EP; Beuron, F; Morris, E; Vannini, A (2018-01)
      RNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. The initiation of gene ...
    • Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor. 

      da Fonseca, PCA; Kong, EH; Zhang, Z; Schreiber, A; Williams, MA; Morris, EP; Barford, D (2011-02)
      The ubiquitylation of cell-cycle regulatory proteins by the large multimeric anaphase-promoting complex (APC/C) controls sister chromatid segregation and the exit from mitosis. Selection of APC/C targets is achieved through ...
    • Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling. 

      Wigington, CP; Roy, J; Damle, NP; Yadav, VK; Blikstad, C; Resch, E; Wong, CJ; Mackay, DR; Wang, JT; Krystkowiak, I; Bradburn, DA; Tsekitsidou, E; Hong, SH; Kaderali, MA; Xu, S-L; Stearns, T; Gingras, A-C; Ullman, KS; Ivarsson, Y; Davey, NE; Cyert, MS (2020-07-08)
      Short linear motifs (SLiMs) drive dynamic protein-protein interactions essential for signaling, but sequence degeneracy and low binding affinities make them difficult to identify. We harnessed unbiased systematic approaches ...