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Desmoplastic small round cell tumor: evaluation of reverse transcription-polymerase chain reaction and fluorescence in situ hybridization as ancillary molecular diagnostic techniques.

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Publication Date
2017-11
ICR Author
Fisher, Cyril
Jones, Robin
Thway, Khin
Marsden,
Author
Mohamed, M
Gonzalez, D
Fritchie, KJ
Swansbury, J
Wren, D
Benson, C
Jones, RL
Fisher, C
Thway, K
Type
Journal Article
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Abstract
Desmoplastic small round cell tumor (DSRCT) is a rare, biologically aggressive soft tissue neoplasm of uncertain differentiation, most often arising in the abdominal and pelvic cavities of adolescents and young adults with a striking male predominance. Histologically, it is characterized by islands of uniform small round cells in prominent desmoplastic stroma, and it has a polyimmunophenotypic profile, typically expressing WT1 and cytokeratin, desmin, and neural/neuroendocrine differentiation markers to varying degrees. Tumors at other sites and with variant morphology are more rarely described. DSRCT is associated with a recurrent t(11;22)(p13;q12) translocation, leading to the characteristic EWSR1-WT1 gene fusion. Fluorescence in situ hybridization (FISH), to detect EWSR1 rearrangement, and reverse transcription-polymerase chain reaction (RT-PCR) to assess for EWSR1-WT1 fusion transcripts are routine diagnostic ancillary tools. We present a large institutional comparative series of FISH and RT-PCR for DSRCT diagnosis. Twenty-six specimens (from 25 patients) histologically diagnosed as DSRCT were assessed for EWSR1 rearrangement and EWSR1-WT1 fusion transcripts. Of these 26 specimens, 24 yielded positive results with either FISH or RT-PCR or both. FISH was performed in 23 samples, with EWSR1 rearrangement seen in 21 (91.3%). RT-PCR was performed in 18 samples, of which 13 (72.2%) harbored EWSR1-WT1 fusion transcripts. The sensitivity of FISH in detecting DSRCT was 91.3%, and that of RT-PCR was 92.8% following omission of four technical failures. Therefore, both methods are comparable in terms of sensitivity. FISH is more sensitive if technical failures for RT-PCR are taken into account, and RT-PCR is more specific in confirming DSRCT. Both methods complement each other by confirming cases that the other method may not. In isolation, FISH is a relatively non-specific diagnostic adjunct due to the number of different neoplasms that can harbor EWSR1 rearrangement, such as Ewing sarcoma. However, in cases with appropriate morphology and a typical pattern of immunostaining, FISH is confirmatory of the diagnosis.
URL
https://repository.icr.ac.uk/handle/internal/1094
Collections
  • Clinical Studies
Version of record
10.1007/s00428-017-2207-y
Subject
Humans
Calmodulin-Binding Proteins
RNA-Binding Proteins
RNA-Binding Protein EWS
Oncogene Proteins, Fusion
In Situ Hybridization, Fluorescence
Reverse Transcriptase Polymerase Chain Reaction
Adolescent
Adult
Middle Aged
Child
Female
Male
Young Adult
Desmoplastic Small Round Cell Tumor
Research team
Sarcoma Clinical Trials (R Jones)
Language
eng
Date accepted
2017-07-18
License start date
2017-11
Citation
Virchows Archiv : an international journal of pathology, 2017, 471 (5), pp. 631 - 640

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