Publications Repository

Publications Repository

View item 
  •   Home
  • ICR Divisions
  • Closed Research Teams
  • View item
  • Home
  • ICR Divisions
  • Closed Research Teams
  • View item
JavaScript is disabled for your browser. Some features of this site may not work without it.

GRASP65 and GRASP55 Sequentially Promote the Transport of C-terminal Valine-bearing Cargos to and through the Golgi Complex

Thumbnail
Date
2009-12
ICR Author
Marra, PierFrancesco
Type
Journal Article
Metadata
Show full item record
Abstract
The Golgi matrix proteins GRASP65 and GRASP55 have recognized roles in maintaining the architecture of the Golgi complex, in mitotic progression and in unconventional protein secretion whereas, surprisingly, they have been shown to be dispensable for the transport of commonly used reporter cargo proteins along the secretory pathway. However, it is becoming increasingly clear that many trafficking machineries operate in a cargo-specific manner, thus we have investigated whether GRASPs may control the trafficking of selected classes of cargo. We have taken into consideration the C-terminal valine-bearing receptors CD8 alpha and Frizzled4 that we show bind directly to the PSD95-DlgA-zo-1 (PDZ) domains of GRASP65 and GRASP55. We demonstrate that both GRASPs are needed sequentially for the efficient transport to and through the Golgi complex of these receptors, thus highlighting a novel role for the GRASPs in membrane trafficking. Our results open new perspectives for our understanding of the regulation of surface expression of a class of membrane proteins, and suggests the causal mechanisms of a dominant form of autosomal human familial exudative vitreoretinopathy that arises from the Frizzled4 mutation involving its C-terminal valine.
URI
https://repository.icr.ac.uk/handle/internal/1724
Collections
  • Closed Research Teams
Subject
ENDOPLASMIC-RETICULUM EXPORT; CELL-SURFACE; INTERMEDIATE COMPARTMENT; STRUCTURAL INTEGRITY; MEMBRANE-PROTEINS; SELECTIVE EXPORT; MAMMALIAN-CELLS; REGULATES GOLGI; PROTGF-ALPHA; ER EXPORT
Research team
Oncogene
Language
eng
License start date
2009-12
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (50), pp. 34849 - 34860
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Browse

All of ICR repositoryICR DivisionsBy issue dateAuthorsTitlesPublication TypesThis collectionBy issue dateAuthorsTitlesPublication Types
  • Login
  • Registered office: The Institute of Cancer Research, 123 Old Brompton Road, London, SW7 3RP
    A Charity, Not for Profit. Company Limited by Guarantee.
    Registered in England No. 534147. VAT Registration No. GB 849 0581 02.