Show simple item record

dc.contributor.authorLorentzen, A
dc.contributor.authorBamber, J
dc.contributor.authorSadok, A
dc.contributor.authorElson-Schwab, I
dc.contributor.authorMarshall, CJ
dc.date.accessioned2018-06-06T09:29:18Z
dc.date.issued2011-04
dc.identifier.citationJournal of cell science, 2011, 124 (Pt 8), pp. 1256 - 1267
dc.identifier.issn0021-9533
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/1732
dc.identifier.eissn1477-9137
dc.identifier.doi10.1242/jcs.074849
dc.description.abstractMelanoma cells can switch between an elongated mesenchymal-type and a rounded amoeboid-type migration mode. The rounded 'amoeboid' form of cell movement is driven by actomyosin contractility resulting in membrane blebbing. Unlike elongated A375 melanoma cells, rounded A375 cells do not display any obvious morphological front-back polarisation, although polarisation is thought to be a prerequisite for cell movement. We show that blebbing A375 cells are polarised, with ezrin (a linker between the plasma membrane and actin cytoskeleton), F-actin, myosin light chain, plasma membrane, phosphatidylinositol (4,5)-bisphosphate and β1-integrin accumulating at the cell rear in a uropod-like structure. This structure does not have the typical protruding shape of classical leukocyte uropods, but, as for those structures, it is regulated by protein kinase C. We show that the ezrin-rich uropod-like structure (ERULS) is an inherent feature of polarised A375 cells and not a consequence of cell migration, and is necessary for cell invasion. Furthermore, we demonstrate that membrane blebbing is reduced at this site, leading to a model in which the rigid ezrin-containing structure determines the direction of a moving cell through localised inhibition of membrane blebbing.
dc.formatPrint
dc.format.extent1256 - 1267
dc.languageeng
dc.language.isoeng
dc.subjectCells
dc.subjectCell Line, Tumor
dc.subjectCell Membrane
dc.subjectHumans
dc.subjectMelanoma
dc.subjectCytoskeletal Proteins
dc.subjectCell Movement
dc.subjectCell Polarity
dc.titleAn ezrin-rich, rigid uropod-like structure directs movement of amoeboid blebbing cells.
dc.typeJournal Article
rioxxterms.versionofrecord10.1242/jcs.074849
rioxxterms.licenseref.startdate2011-04
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfJournal of cell science
pubs.issuePt 8
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Ultrasound & Optical Imaging
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Ultrasound & Optical Imaging
pubs.publication-statusPublished
pubs.volume124
pubs.embargo.termsNot known
icr.researchteamOncogeneen_US
icr.researchteamUltrasound & Optical Imagingen_US
dc.contributor.icrauthorMarshall, Christopheren
dc.contributor.icrauthorBamber, Jeffreyen


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following collection(s)

Show simple item record