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dc.contributor.authorDas, Aen_US
dc.contributor.authorGajendra, Sen_US
dc.contributor.authorFalenta, Ken_US
dc.contributor.authorOudin, MJen_US
dc.contributor.authorPeschard, Pen_US
dc.contributor.authorFeng, Sen_US
dc.contributor.authorWu, Ben_US
dc.contributor.authorMarshall, CJen_US
dc.contributor.authorDoherty, Pen_US
dc.contributor.authorGuo, Wen_US
dc.contributor.authorLalli, Gen_US
dc.date.accessioned2018-06-06T10:06:12Z
dc.date.issued2014-02en_US
dc.identifier.citationJournal of cell science, 2014, 127 (Pt 3), pp. 686 - 699en_US
dc.identifier.issn0021-9533en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/1740
dc.identifier.eissn1477-9137en_US
dc.identifier.doi10.1242/jcs.145037en_US
dc.description.abstractCell polarization is essential for neuronal development in both the embryonic and postnatal brain. Here, using primary cultures, in vivo postnatal electroporation and conditional genetic ablation, we show that the Ras-like small GTPase RalA and its effector, the exocyst, regulate the morphology and polarized migration of neural progenitors derived from the subventricular zone, a major neurogenic niche in the postnatal brain. Active RalA promotes the direct binding between the exocyst subunit Exo84 and the PDZ domain of Par6 through a non-canonical PDZ-binding motif. Blocking the Exo84-Par6 interaction impairs polarization in postnatal neural progenitors and cultured embryonic neurons. Our results provide the first in vivo characterization of RalA function in the mammalian brain and highlight a novel molecular mechanism for cell polarization. Given that the exocyst and the Par complex are conserved in many tissues, the functional significance of their interaction and its regulation by RalA are likely to be important in a wide range of polarization events.en_US
dc.formatPrint-Electronicen_US
dc.format.extent686 - 699en_US
dc.languageengen_US
dc.language.isoengen_US
dc.subjectBrainen_US
dc.subjectNeuronsen_US
dc.subjectAnimalsen_US
dc.subjectral GTP-Binding Proteinsen_US
dc.subjectAdaptor Proteins, Signal Transducingen_US
dc.subjectVesicular Transport Proteinsen_US
dc.subjectSignal Transductionen_US
dc.subjectCell Polarityen_US
dc.subjectProtein Bindingen_US
dc.subjectPDZ Domainsen_US
dc.subjectNeurogenesisen_US
dc.subjectPrimary Cell Cultureen_US
dc.titleRalA promotes a direct exocyst-Par6 interaction to regulate polarity in neuronal development.en_US
dc.typeJournal Article
rioxxterms.versionofrecord10.1242/jcs.145037en_US
rioxxterms.licenseref.startdate2014-02en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfJournal of cell scienceen_US
pubs.issuePt 3en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Oncogene
pubs.volume127en_US
pubs.embargo.termsNot knownen_US
pubs.oa-locationhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007768/en_US
icr.researchteamOncogeneen_US
dc.contributor.icrauthorMarshall, Christopheren_US


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