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dc.contributor.authorDas, A
dc.contributor.authorGajendra, S
dc.contributor.authorFalenta, K
dc.contributor.authorOudin, MJ
dc.contributor.authorPeschard, P
dc.contributor.authorFeng, S
dc.contributor.authorWu, B
dc.contributor.authorMarshall, CJ
dc.contributor.authorDoherty, P
dc.contributor.authorGuo, W
dc.contributor.authorLalli, G
dc.date.accessioned2018-06-06T10:06:12Z
dc.date.issued2014-02
dc.identifier.citationJournal of cell science, 2014, 127 (Pt 3), pp. 686 - 699
dc.identifier.issn0021-9533
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/1740
dc.identifier.eissn1477-9137
dc.identifier.doi10.1242/jcs.145037
dc.description.abstractCell polarization is essential for neuronal development in both the embryonic and postnatal brain. Here, using primary cultures, in vivo postnatal electroporation and conditional genetic ablation, we show that the Ras-like small GTPase RalA and its effector, the exocyst, regulate the morphology and polarized migration of neural progenitors derived from the subventricular zone, a major neurogenic niche in the postnatal brain. Active RalA promotes the direct binding between the exocyst subunit Exo84 and the PDZ domain of Par6 through a non-canonical PDZ-binding motif. Blocking the Exo84-Par6 interaction impairs polarization in postnatal neural progenitors and cultured embryonic neurons. Our results provide the first in vivo characterization of RalA function in the mammalian brain and highlight a novel molecular mechanism for cell polarization. Given that the exocyst and the Par complex are conserved in many tissues, the functional significance of their interaction and its regulation by RalA are likely to be important in a wide range of polarization events.
dc.formatPrint-Electronic
dc.format.extent686 - 699
dc.languageeng
dc.language.isoeng
dc.subjectBrain
dc.subjectNeurons
dc.subjectAnimals
dc.subjectral GTP-Binding Proteins
dc.subjectAdaptor Proteins, Signal Transducing
dc.subjectVesicular Transport Proteins
dc.subjectSignal Transduction
dc.subjectCell Polarity
dc.subjectProtein Binding
dc.subjectPDZ Domains
dc.subjectNeurogenesis
dc.subjectPrimary Cell Culture
dc.titleRalA promotes a direct exocyst-Par6 interaction to regulate polarity in neuronal development.
dc.typeJournal Article
rioxxterms.versionofrecord10.1242/jcs.145037
rioxxterms.licenseref.startdate2014-02
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfJournal of cell science
pubs.issuePt 3
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene
pubs.publication-statusPublished
pubs.volume127
pubs.embargo.termsNot known
pubs.oa-locationhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007768/
icr.researchteamOncogeneen_US
dc.contributor.icrauthorMarshall, Christopheren


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