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dc.contributor.authorFontebasso, Y
dc.contributor.authorEtheridge, TJ
dc.contributor.authorOliver, AW
dc.contributor.authorMurray, JM
dc.contributor.authorCarr, AM
dc.date.accessioned2018-06-08T11:45:38Z
dc.date.issued2013-12
dc.identifier.citationDNA repair, 2013, 12 (12), pp. 1011 - 1023
dc.identifier.issn1568-7864
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/1764
dc.identifier.eissn1568-7856
dc.identifier.doi10.1016/j.dnarep.2013.10.003
dc.description.abstractDNA interstrand cross-links (ICLs) represent a physical barrier to the progression of cellular machinery involved in DNA metabolism. Thus, this type of adduct represents a serious threat to genomic stability and as such, several DNA repair pathways have evolved in both higher and lower eukaryotes to identify this type of damage and restore the integrity of the genetic material. Human cells possess a specialized ICL-repair system, the Fanconi anemia (FA) pathway. Conversely yeasts rely on the concerted action of several DNA repair systems. Recent work in higher eukaryotes identified and characterized a novel conserved FA component, FAN1 (Fanconi anemia-associated nuclease 1, or FANCD2/FANCI-associated nuclease 1). In this study, we characterize Fan1 in the yeast Schizosaccharomyces pombe. Using standard genetics, we demonstrate that Fan1 is a key component of a previously unidentified ICL-resolution pathway. Using high-throughput synthetic genetic arrays, we also demonstrate the existence of a third pathway of ICL repair, dependent on the SUMO E3 ligase Pli1. Finally, using sequence-threaded homology models, we predict and validate key residues essential for Fan1 activity in ICL repair.
dc.formatPrint-Electronic
dc.format.extent1011 - 1023
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectSchizosaccharomyces
dc.subjectDNA Damage
dc.subjectEsterases
dc.subjectEndodeoxyribonucleases
dc.subjectLigases
dc.subjectUbiquitin-Protein Ligases
dc.subjectSchizosaccharomyces pombe Proteins
dc.subjectEvolution, Molecular
dc.subjectSignal Transduction
dc.subjectDNA Repair
dc.subjectRecombination, Genetic
dc.subjectProtein Structure, Tertiary
dc.subjectSequence Homology
dc.subjectModels, Molecular
dc.subjectHigh-Throughput Screening Assays
dc.subjectMutation Rate
dc.titleThe conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
dc.typeJournal Article
dcterms.dateAccepted2013-10-07
rioxxterms.versionofrecord10.1016/j.dnarep.2013.10.003
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2013-12
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfDNA repair
pubs.issue12
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.publication-statusPublished
pubs.volume12en_US
pubs.embargo.termsNot known
pubs.oa-locationhttps://ac.els-cdn.com/S1568786413002425/1-s2.0-S1568786413002425-main.pdf?_tid=f2098b31-a780-4905-828a-98edfb825ae5&acdnat=1528458366_b0c8b54003206acde0dcc0f3fef5724c
dc.contributor.icrauthorFontebasso, Yen


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