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dc.identifier.citationJOURNAL OF CELL BIOLOGY, 2011, 194 pp. 789 - 805
dc.description.abstractAlthough a large number of actin-binding proteins and their regulators have been identified through classical approaches, gaps in our knowledge remain. Here, we used genome-wide RNA interference as a systematic method to define metazoan actin regulators based on visual phenotype. Using comparative screens in cultured Drosophila and human cells, we generated phenotypic profiles for annotated actin regulators together with proteins bearing predicted actin-binding domains. These phenotypic clusters for the known metazoan "actinome" were used to identify putative new core actin regulators, together with a number of genes with conserved but poorly studied roles in the regulation of the actin cytoskeleton, several of which we studied in detail. This work suggests that although our search for new components of the core actin machinery is nearing saturation, regulation at the level of nuclear actin export, RNA splicing, ubiquitination, and other upstream processes remains an important but unexplored frontier of actin biology.
dc.format.extent789 - 805
dc.titleComparative RNAi screening identifies a conserved core metazoan actinome by phenotype
dc.typeJournal Article
rioxxterms.typeJournal Article/Review
pubs.notesISI Document Delivery No.: 816LU Times Cited: 2 Cited Reference Count: 91 Rohn, Jennifer L. Sims, David Liu, Tao Fedorova, Marina Schoeck, Frieder Dopie, Joseph Vartiainen, Maria K. Kiger, Amy A. Perrimon, Norbert Baum, Buzz Wellcome Trust; Academy of Finland; University of Helsinki; Sigrid Juselius foundation; Helsinki Graduate Program in Biotechnology and Molecular Biology; Royal Society; EMBO; UCL; Cancer Research UK J.L. Rohn was funded by a fellowship from the Wellcome Trust. M.K. Vartiainen was funded by Academy of Finland, University of Helsinki Research Funds, and Sigrid Juselius foundation. J. Dopie was funded by a fellowship from the Helsinki Graduate Program in Biotechnology and Molecular Biology. N. Perrimon is an investigator of the Howard Hughes Medical Institute. B. Baum was funded by the Royal Society, the EMBO YIP program, UCL, and Cancer Research UK. Rockefeller univ press New york keywords: e3 ubiquitin ligase f-box proteins nuclear actin cell polarity saccharomyces-cerevisiae drosophila-melanogaster epithelial-cells wave complex transcription merlin
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dc.contributor.icrauthorSims, Daviden

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