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PACE-1, a novel protein that interacts with the C-terminal domain of ezrin.

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Publication Date
2003-04
ICR Author
Isacke, Clare
Author
Sullivan, A
Uff, CR
Isacke, CM
Thorne, RF
Type
Journal Article
Metadata
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Abstract
The ERM proteins (ezrin, radixin, moesin) together with merlin comprise a subgroup of the band 4.1 superfamily. These proteins act as membrane cytoskeletal linker proteins mediating interactions between the cytoplasmic domains of transmembrane proteins and actin. To better understand how the ERM proteins function to regulate these junctional complexes, a yeast 2-hybrid screen was undertaken using ezrin as a bait. We describe here the identification and cloning of a novel protein, PACE-1, which binds to the C-terminal domain of ezrin. Characterization of PACE-1 in human breast cancer cell lines demonstrates it to have two distinct intracellular localizations. A proportion of the protein is associated with the cytoplasmic face of the Golgi apparatus. This distribution is dependent upon the presence of the PACE-1 N-terminal myristoylation consensus sequence but is not dependent on an association with ezrin. In contrast, PACE-1 colocalises with ezrin in the lamellipodia, where ezrin has a role in cell spreading and motility. A notable feature of PACE-1 is the presence of a putative N-terminal kinase domain; however, in biochemical assays PACE-1 was shown to have associated rather than intrinsic kinase activity. Together these data suggest that PACE-1 may play a role in regulating cell adhesion/migration complexes in migrating cells.
URL
https://repository.icr.ac.uk/handle/internal/1832
Collections
  • Breast Cancer Research
Version of record
10.1016/s0014-4827(02)00054-x
Subject
COS Cells
Tumor Cells, Cultured
Cell Membrane
Pseudopodia
Golgi Apparatus
Eukaryotic Cells
Animals
Humans
Fatty Acids, Monounsaturated
Cytoskeletal Proteins
Phosphoproteins
DNA, Complementary
Cloning, Molecular
Cell Adhesion
Cell Movement
Amino Acid Sequence
Base Sequence
Protein Structure, Tertiary
Protein Binding
Molecular Sequence Data
Research team
Molecular Cell Biology
Language
eng
License start date
2003-04
Citation
Experimental cell research, 2003, 284 (2), pp. 224 - 238

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