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dc.date.accessioned2018-06-11T14:39:39Z
dc.date.issued2002-12
dc.identifier12
dc.identifier.citationJOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2002, 50 pp. 1671 - 1676
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/1834
dc.description.abstractExpression of P-glycoprotein in human cerebral cortex microvessels. P-Glycoprotein (P-gp) is an ATP-dependent efflux transporter that extrudes non-polar molecules, including cytotoxic substances and drugs, from the cells. It was initially found in cancer cells and then was shown to be a normal component of complex transport systems working at the blood-brain barrier (BBB). Previous studies have demonstrated that, in the brain, P-gp is localized on the luminal plasmalemma of BBB endothelial cells and that it may interact with the caveolar compartment of these cells. The aim of this study was to identify the site of cellular expression of P-gp in human brain in situ and to morphologically determine whether an association may exist between P-gp and caveolin-1, a structural and functional protein of the caveolar frame. The study was carried out on human cerebral cortex by immunoconfocal microscopy with antibodies to both P-gp and caveolin-1. The results show that P-gp marks the microvessels of the cortex and that the transporter is localized in the luminal endothelial compartment, where it co-localizes with caveolin-1. The demonstration of this co-localization of P-gp with caveolin-1 contributes a morphological backing to biochemical studies on P-gp/caveolin-1 relationships and leads us to suggest that interactions between these molecules may occur at the BBB endothelia.
dc.format.extent1671 - 1676
dc.languageeng
dc.language.isoeng
dc.titleExpression of P-glycoprotein in human cerebral cortex microvessels.
dc.typeJournal Article
rioxxterms.licenseref.startdate2002-12
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfJOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
pubs.noteskeywords: P-glycoprotein; caveolin-1; human brain microvessels; blood-brain barrier; immunoconfocal microscopy; BLOOD-BRAIN-BARRIER; MULTIDRUG-RESISTANCE; ENDOTHELIAL-CELLS; CELLULAR-LOCALIZATION; CAPILLARIES; CAVEOLAE; TRANSPORT; TISSUES; DRUGS
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Molecular Cell Biology
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Molecular Cell Biology
pubs.volume50
pubs.embargo.termsNot known
icr.researchteamMolecular Cell Biologyen_US
dc.contributor.icrauthorRobertson, Daviden


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