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Genomic gain and over expression of CCL2 correlate with vascular invasion in stage I non-seminomatous testicular germ-cell tumours.

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Publication Date
2011-08
ICR Author
Shipley, Janet
Huddart, Robert
Goddard, Neil Christopher
Author
Gilbert, DC
Chandler, I
Summersgill, B
McIntyre, A
Missiaglia, E
Goddard, NC
Huddart, RA
Shipley, J
Type
Journal Article
Metadata
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Abstract
Testicular germ-cell tumours (TGCT) are the most frequent solid tumour to affect young Caucasian adult males and have increased in incidence over recent decades. In clinical stage I non-seminomas, (NSGCT) histological vascular invasion (VI) is a prognostic factor for metastatic relapse. Using array comparative genomic hybridization, we have previously shown that the presence of VI is associated with gain of a region at 17q12, containing a cluster of genes encoding inflammatory cytokines. We here confirm this finding using fluorescence in situ hybridization (FISH) demonstrating gain in 12 out of 42 (29%) assessable samples. Interrogation of previously published expression microarray data suggests that of the genes contained within this region, CCL2 [monocyte chemoattractant protein 1 (MCP1)] is frequently overexpressed in TGCT. Immunohistochemistry confirms this finding in a collection of 67 clinical stage I NSGCT, demonstrating an association with the presence of VI (p=0.049) that was not seen with VEGF-A, MMP2 or MMP9, although all were frequently expressed. This work gives further insight into the mechanisms involved in invasion in this tumour type, which may ultimately have implications for the management of patients with stage I disease.
URL
https://repository.icr.ac.uk/handle/internal/1903
Collections
  • Cancer Therapeutics
  • Molecular Pathology
  • Radiotherapy and Imaging
Version of record
10.1111/j.1365-2605.2011.01161.x
Subject
Chromosomes, Human, Pair 17
Humans
Neoplasms, Germ Cell and Embryonal
Testicular Neoplasms
Neovascularization, Pathologic
Vascular Endothelial Growth Factor A
Neoplasm Staging
Prognosis
Oligonucleotide Array Sequence Analysis
In Situ Hybridization, Fluorescence
Genome, Human
Adolescent
Adult
Middle Aged
Male
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Chemokine CCL2
Biomarkers, Tumor
Research team
Sarcoma Molecular Pathology
Clinical Academic Radiotherapy (Huddart)
Language
eng
License start date
2011-08
Citation
International journal of andrology, 2011, 34 (4 Pt 2), pp. e114 - e121

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