A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator
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A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator Telomere capping is the essential function of telomeres. To identify new genes involved in telomere capping, we carried out a genome-wide screen in Saccharomyces cerevisiae for suppressors of cdc13-1, an allele of the telomere-capping protein Cdc13. We report the identification of five novel suppressors, including the previously uncharacterized gene YML036W, which we name CGI121. Cgi121 is part of a conserved protein complex-the KEOPS complex-containing the protein kinase Bud32, the putative peptidase Kae1, and the uncharacterized protein Gon7. Deletion of CGI121 suppresses cdc13-1 via the dramatic reduction in ssDNA levels that accumulate in cdc13-1 cgi121 mutants. Deletion of BUD32 or other KEOPS components leads to short telomeres and at failure to add telomeres de novo to DNA double-strand breaks. Our results therefore indicate that the KEOPS complex promotes both telomere uncapping and telomere elongation.
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Single-stranded-dna de-novo telomere saccharomyces-cerevisiae chromosome ends checkpoint pathways yeast pid261/bud32 binding protein cdc13-1 mutants in-vivo damage
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CELL, 2006, 124 (6), pp. 1155 - 1168