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dc.contributor.authorBarber, KE
dc.contributor.authorFord, AM
dc.contributor.authorHarris, RL
dc.contributor.authorHarrison, CJ
dc.contributor.authorMoorman, AV
dc.date.accessioned2018-07-11T10:15:47Z
dc.date.issued2004-11
dc.identifier.citationGenes, chromosomes & cancer, 2004, 41 (3), pp. 266 - 271
dc.identifier.issn1045-2257
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2027
dc.identifier.eissn1098-2264en_US
dc.identifier.doi10.1002/gcc.20082en_US
dc.description.abstractRearrangements involving the MLL gene at 11q23 occur in a clinically relevant subgroup of patients with acute lymphoblastic leukemia (ALL) at all ages, and therefore their accurate identification at diagnosis is important. It has become commonplace to screen ALL patients for rearrangements of MLL using a dual-color fluorescence in situ hybridization (FISH) assay. We report on 12 ALL patients with an unusual FISH result consisting of the following signal pattern: one 5' green, no 3' red, and one/two fusion signals. This configuration is consistent with a MLL translocation and simultaneous deletion of 3' MLL-a well-established phenomenon-which has been interpreted as a positive result. G-banded and complementary metaphase FISH analyses confirmed an 11q23/MLL translocation in 8 of the 12 cases, whereas in one case, the identification of a del(11)(q23) was restricted to G-banded analysis only. In three cases, an MLL rearrangement was excluded by extensive FISH analysis and/or Southern blotting. In conclusion, the loss of the 3' MLL signal should not be assumed to be the result of a concurrent translocation and deletion event, and such aberrant FISH signal patterns should be investigated further by alternative methods for determining their MLL status.
dc.formatPrint
dc.format.extent266 - 271
dc.languageeng
dc.language.isoeng
dc.subjectChromosomes, Human, Pair 11
dc.subjectHumans
dc.subjectTranslocation, Genetic
dc.subjectHistone-Lysine N-Methyltransferase
dc.subjectDNA-Binding Proteins
dc.subjectTranscription Factors
dc.subjectBlotting, Southern
dc.subjectChromosome Banding
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectKaryotyping
dc.subjectMetaphase
dc.subjectGene Deletion
dc.subjectProto-Oncogenes
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectInfant
dc.subjectFemale
dc.subjectMale
dc.subjectMyeloid-Lymphoid Leukemia Protein
dc.subjectStatistics as Topic
dc.subjectPrecursor Cell Lymphoblastic Leukemia-Lymphoma
dc.titleMLL translocations with concurrent 3' deletions: interpretation of FISH results.
dc.typeJournal Article
rioxxterms.versionofrecord10.1002/gcc.20082
rioxxterms.licenseref.startdate2004-11en_US
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfGenes, chromosomes & cancer
pubs.issue3
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Biology of Childhood Leukaemia
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Biology of Childhood Leukaemia
pubs.publication-statusPublished
pubs.volume41en_US
pubs.embargo.termsNot known
icr.researchteamBiology of Childhood Leukaemiaen_US
dc.contributor.icrauthorFord, Anthonyen


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