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A Kinetic Test Characterizes Kinase Intramolecular and Intermolecular Autophosphorylation Mechanisms

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Date
2013-07-02
ICR Author
Dodson, Charlotte
Bayliss, Richard
Author
Dodson, CA
Yeoh, S
Haq, T
Bayliss, R
Type
Journal Article
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Abstract
Many protein kinases catalyze their own activation by autophosphorylation. The mechanism of this is generally considered to be intermolecular and similar to that used in substrate phosphorylation. We derived the kinetic signatures of the four simplest autophosphorylation reactions and developed a test to determine the autoactivation mechanism of individual kinases. Whereas autophosphorylation of Nek7 and Plk4 occurred through an intermolecular mechanism, the kinases Aurora-A and Chk2 followed an intramolecular mechanism. Autophosphorylation of Aurora-A was accelerated in the presence of its protein activator TPX2. Nek9, the binding partner for Nek7, had a concentration-dependent effect such that low amounts enhanced autoactivation of Nek7 and high amounts were inhibitory. A structural model of Aurora-A undergoing autophosphorylation confirmed that an intramolecular mechanism is physically possible, and provided an explanation for how TPX2 could stimulate both autophosphorylation and substrate phosphorylation. The distinct mechanisms of autoactivation have consequences for cellular regulation because each molecule of a kinase that undergoes intramolecular autophosphorylation is activated individually, whereas the activity of kinases that undergo intermolecular autophosphorylation can be rapidly self-amplified in the cell. Local control of individual molecules, such as Aurora-A, may be particularly advantageous for a kinase with multiple, distinct cellular roles.
URI
https://repository.icr.ac.uk/handle/internal/2269
DOI
https://doi.org/10.1126/scisignal.2003910
Collections
  • Other ICR Research
Language
eng
License start date
2013-07-02
Citation
SCIENCE SIGNALING, 2013, 6
Publisher
AMER ASSOC ADVANCEMENT SCIENCE

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