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dc.contributor.authorNorris, RLGen_US
dc.contributor.authorPaul, Men_US
dc.contributor.authorGeorge, Ren_US
dc.contributor.authorMoore, Aen_US
dc.contributor.authorPinkerton, Ren_US
dc.contributor.authorHaywood, Aen_US
dc.contributor.authorCharles, Ben_US
dc.date.accessioned2018-08-08T10:28:45Z
dc.date.issued2012-06-01en_US
dc.identifier.citationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2012, 898 pp. 136 - 140en_US
dc.identifier.issn1570-0232en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2302
dc.identifier.doi10.1016/j.jchromb.2012.04.003en_US
dc.description.abstractBackground: Glutathione is the principal non-protein tripeptide thiol present in most mammalian cells and plays an important role in the redox status of biological systems. The accurate assessment of reduced glutathione (GSH) status as a reliable index of oxidative stress is of research and clinical significance. GSH undergoes rapid oxidation after sample collection and this presents a challenge. Methods: Validation of an HPLC-MS/MS assay is reported. Storage stability using four variants of a methanolic precipitation with addition of stable isotope internal standard at collection is compared to L-serine borate/EDTA with perchloric acid precipitation (SBPE). Results: Precipitation with methanol and addition of stable isotope on sample collection, combined with storage in solution at -70 degrees C showed superior storage stability to SBPE and other variants of the methanolic precipitation method up to 99 days. Conclusions: The combination of stable isotope with methanolic precipitation at collection, with assay by HPLC-MS/MS provides superior results after storage of whole blood samples for at least 99 days. (C) 2012 Elsevier B.V. All rights reserved.en_US
dc.format.extent136 - 140en_US
dc.languageEnglishen_US
dc.language.isoEnglishen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.titleA stable-isotope HPLC-MS/MS method to simplify storage of human whole blood samples for glutathione assayen_US
dc.typeJournal Article
rioxxterms.versionofrecord10.1016/j.jchromb.2012.04.003en_US
rioxxterms.licenseref.startdate2012-06-01en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCESen_US
pubs.notesaffiliation: Norris, RLG (Reprint Author), Australian Ctr Paediat Pharmacokinet, Mater Med Res Inst, Raymond Terrace, Brisbane, Qld 4101, Australia. Norris, R. L. G.; George, R., Australian Ctr Paediat Pharmacokinet, Mater Med Res Inst, Brisbane, Qld 4101, Australia. Norris, R. L. G.; Paul, M.; Haywood, A., Griffith Univ, Sch Pharm, Gold Coast 4222, Australia. Norris, R. L. G.; Charles, B., Univ Queensland, Sch Pharm, Brisbane, Qld 4072, Australia. Moore, A., Inst Canc Res, Paediat Sect, Sutton, Surrey, England. Moore, A., Royal Marsden NHS Fdn Trust, Sutton, Surrey, England. Pinkerton, R., Royal Childrens Hosp, Queensland Childrens Canc Ctr, Brisbane, Qld, Australia. keywords: Glutathione; GSH; Assay; Stability; HPLC-MS/MS keywords-plus: PERFORMANCE LIQUID-CHROMATOGRAPHY; OXIDIZED GLUTATHIONE; MASS-SPECTROMETRY; PLASMA; CELLS research-areas: Biochemistry & Molecular Biology; Chemistry web-of-science-categories: Biochemical Research Methods; Chemistry, Analytical author-email: [email protected] researcherid-numbers: Moore, Andrew/I-6275-2012 orcid-numbers: Moore, Andrew/0000-0001-8062-1779 number-of-cited-references: 12 times-cited: 11 usage-count-last-180-days: 2 usage-count-since-2013: 22 journal-iso: J. Chromatogr. B doc-delivery-number: 956NZ unique-id: ISI:000305097700017 da: 2018-08-08en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.volume898en_US
pubs.embargo.termsNot knownen_US
dc.contributor.icrauthorMoore, Andrewen_US


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