Publications Repository

Publications Repository

View Item 
  •   Home
  • ICR Divisions
  • Closed Research Teams
  • View Item
  • Home
  • ICR Divisions
  • Closed Research Teams
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Generation of Simplified Protein Raman Spectra Using Three-Color Picosecond Coherent Anti-Stokes Raman Spectroscopy

Thumbnail
Publication Date
2010-09-23
ICR Author
Willison, Keith
Author
Donaldson, PM
Willison, KR
Klug, DR
Type
Journal Article
Metadata
Show full item record
Abstract
The well-known and prominent marker bands of aromatic amino acids in Raman spectra of protein and peptide films are revisited in the frequency and time domains using three-color picosecond coherent anti-Stokes Raman spectroscopy (CARS). We show here that control of the probe delay allows the narrow width/long lifetime states to be observed free not only from nonresonant background and fluorescence contamination but also free from the spectral congestion that arises from the complex background of spectrally broader (shorter lifetime) vibrational modes. The reasonable limits of detection obtained indicate that such CARS methods may be useful for quantitative analysis of protein composition.
URL
https://repository.icr.ac.uk/handle/internal/2362
Collections
  • Closed Research Teams
Version of record
10.1021/jp1061607
Research team
Chromatin Regulation
Language
English
License start date
2010-09-23
Citation
JOURNAL OF PHYSICAL CHEMISTRY B, 2010, 114 pp. 12175 - 12181
Publisher
AMER CHEMICAL SOC

Browse

All of ICR repositoryICR DivisionsIssue dateAuthorsTitlesSubjectsThis collectionIssue dateAuthorsTitlesSubjects

Statistics

Most popular itemsStatistics by countryMost popular authors
  • Login
  • Registered office: The Institute of Cancer Research, 123 Old Brompton Road, London, SW7 3RP
    A Charity, Not for Profit. Company Limited by Guarantee.
    Registered in England No. 534147. VAT Registration No. GB 849 0581 02.