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A microfluidic platform for probing single cell plasma membranes using optically trapped Smart Droplet Microtools (SDMs)

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Publication Date
2009
ICR Author
Willison, Keith
Author
Lanigan, PMP
Ninkovic, T
Chan, K
de Mello, AJ
Willison, KR
Klug, DR
Templer, RH
Neil, MAA
Ces, O
Type
Journal Article
Metadata
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Abstract
We recently introduced a novel platform based upon optically trapped lipid coated oil droplets (Smart Droplet Microtools-SDMs) that were able to form membrane tethers upon fusion with the plasma membrane of single cells. Material transfer from the plasma membrane to the droplet via the tether was seen to occur. Here we present a customised version of the SDM approach based upon detergent coated droplets deployed within a microfluidic format. These droplets are able to differentially solubilise the plasma membrane of single cells with spatial selectivity and without forming membrane tethers. The microfluidic format facilitates separation of the target cells from the bulk SDM population and from downstream analysis modules. Material transfer from the cell to the SDM was monitored by tracking membrane localized EGFP.
URL
https://repository.icr.ac.uk/handle/internal/2399
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  • Closed Research Teams
Version of record
10.1039/b816857a
Research team
Chromatin Regulation
Language
English
License start date
2009
Citation
LAB ON A CHIP, 2009, 9 pp. 1096 - 1101
Publisher
ROYAL SOC CHEMISTRY

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