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dc.contributor.authorAltschuler, GM
dc.contributor.authorWillison, KR
dc.date.accessioned2018-08-30T08:16:40Z
dc.date.issued2008-12-06
dc.identifier29
dc.identifier.citationJOURNAL OF THE ROYAL SOCIETY INTERFACE, 2008, 5 pp. 1391 - 1408
dc.identifier.issn1742-5689
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2416
dc.identifier.eissn1742-5662
dc.identifier.doi10.1098/rsif.2008.0185
dc.description.abstractA free-energy-based approach is used to describe the mechanism through which chaperonin-containing TCP-1 (CCT) folds the. lament-forming cytoskeletal protein actin, which is one of its primary substrates. The experimental observations on the actin folding and unfolding pathways are collated and then re-examined from this perspective, allowing us to determine the position of the CCT intervention on the actin free-energy folding landscape. The essential role for CCT in actin folding is to provide a free-energy contribution from its ATP cycle, which drives actin to fold from a stable, trapped intermediate I-3, to a less stable but now productive folding intermediate I-2. We develop two hypothetical mechanisms for actin folding founded upon concepts established for the bacterial type I chaperonin GroEL and extend them to the much more complex CCT system of eukaryotes. A new model is presented in which CCT facilitates free-energy transfer through direct coupling of the nucleotide hydrolysis cycle to the phases of actin substrate maturation.
dc.format.extent1391 - 1408
dc.languageeng
dc.language.isoeng
dc.publisherROYAL SOC
dc.titleDevelopment of free-energy-based models for chaperonin containing TCP-1 mediated folding of actin
dc.typeJournal Article
rioxxterms.versionofrecord10.1098/rsif.2008.0185
rioxxterms.licenseref.startdate2008-12-06
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfJOURNAL OF THE ROYAL SOCIETY INTERFACE
pubs.notesaffiliation: Willison, KR (Reprint Author), Inst Canc Res, Chester Beatty Labs, Canc Res UK Ctr Cell & Mol Biol, 237 Fulham Rd, London SW3 6JB, England. Altschuler, Gabriel M.; Willison, Keith R., Inst Canc Res, Chester Beatty Labs, Canc Res UK Ctr Cell & Mol Biol, London SW3 6JB, England. Altschuler, Gabriel M., Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England. keywords: actin; chaperonin-containing TCP-1; protein folding; chaperones; free-energy landscapes keywords-plus: EUKARYOTIC CYTOSOLIC CHAPERONIN; NUCLEOTIDE-FREE ACTIN; INDUCED ALLOSTERIC TRANSITIONS; CRYSTAL-STRUCTURE; BETA-ACTIN; DIVALENT-CATION; BINDING-SITES; CYTOPLASMIC CHAPERONIN; MOLECULAR CONFINEMENT; PROTEIN TRANSLOCATION research-areas: Science & Technology - Other Topics web-of-science-categories: Multidisciplinary Sciences author-email: [email protected] funding-acknowledgement: Cancer Research UK, Human Frontiers Science [RGP63/2004]; EPSRC Platform funding-text: This work is funded by Cancer Research UK, Human Frontiers Science Program (RGP63/2004) and an EPSRC Platform Grant. We thank Prof. David Klug for helpful discussions. We thank both the anonymous reviewers of this work for their insightful comments. number-of-cited-references: 126 times-cited: 22 usage-count-last-180-days: 0 usage-count-since-2013: 5 journal-iso: J. R. Soc. Interface doc-delivery-number: 382YY unique-id: ISI:000261642500001 oa: gold_or_bronze da: 2018-08-29
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation
pubs.volume5
pubs.embargo.termsNot known
icr.researchteamChromatin Regulationen_US
dc.contributor.icrauthorWillison, Keithen


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