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Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool

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Date
2008-10-06
ICR Author
Willison, Keith
Author
Lanigan, PMP
Chan, K
Ninkovic, T
Templer, RH
French, PMW
de Mello, AJ
Willison, KR
Parker, PJ
Neil, MAA
Ces, O
Klug, DR
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Type
Journal Article
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Abstract
We present a platform for the spatially selective sampling of the plasma membrane of single cells. Optically trapped lipid-coated oil droplets (smart droplet microtools, SDMs), typically 0.5 5 mu m in size, composed of a hexadecane hydrocarbon core and fusogenic lipid outer coating (mixture of 1,2-dioleoyl-phosphatidylethanolamine and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) were brought into controlled contact with target colon cancer cells leading to the formation of connecting membrane tethers. Material transfer from the cell to the SDM across the membrane tether was monitored by tracking membrane-localized enhanced green fluorescent protein.
URI
https://repository.icr.ac.uk/handle/internal/2424
DOI
https://doi.org/10.1098/rsif.2008.0249.focus
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  • Closed Research Teams
Research team
Chromatin Regulation
Language
eng
License start date
2008-10-06
Citation
JOURNAL OF THE ROYAL SOCIETY INTERFACE, 2008, 5 pp. S161 - S168
Publisher
ROYAL SOC

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