Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool
de Mello, AJ
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We present a platform for the spatially selective sampling of the plasma membrane of single cells. Optically trapped lipid-coated oil droplets (smart droplet microtools, SDMs), typically 0.5 5 mu m in size, composed of a hexadecane hydrocarbon core and fusogenic lipid outer coating (mixture of 1,2-dioleoyl-phosphatidylethanolamine and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) were brought into controlled contact with target colon cancer cells leading to the formation of connecting membrane tethers. Material transfer from the cell to the SDM across the membrane tether was monitored by tracking membrane-localized enhanced green fluorescent protein.
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JOURNAL OF THE ROYAL SOCIETY INTERFACE, 2008, 5 pp. S161 - S168