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Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool

dc.contributor.authorLanigan, PMP
dc.contributor.authorChan, K
dc.contributor.authorNinkovic, T
dc.contributor.authorTempler, RH
dc.contributor.authorFrench, PMW
dc.contributor.authorde Mello, AJ
dc.contributor.authorWillison, KR
dc.contributor.authorParker, PJ
dc.contributor.authorNeil, MAA
dc.contributor.authorCes, O
dc.contributor.authorKlug, DR
dc.date.accessioned2018-08-30T10:23:14Z
dc.date.issued2008-10-06
dc.identifier2
dc.identifier.citationJOURNAL OF THE ROYAL SOCIETY INTERFACE, 2008, 5 pp. S161 - S168
dc.identifier.issn1742-5689
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2424
dc.identifier.doi10.1098/rsif.2008.0249.focus
dc.description.abstractWe present a platform for the spatially selective sampling of the plasma membrane of single cells. Optically trapped lipid-coated oil droplets (smart droplet microtools, SDMs), typically 0.5 5 mu m in size, composed of a hexadecane hydrocarbon core and fusogenic lipid outer coating (mixture of 1,2-dioleoyl-phosphatidylethanolamine and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) were brought into controlled contact with target colon cancer cells leading to the formation of connecting membrane tethers. Material transfer from the cell to the SDM across the membrane tether was monitored by tracking membrane-localized enhanced green fluorescent protein.
dc.format.extentS161 - S168
dc.languageeng
dc.language.isoeng
dc.publisherROYAL SOC
dc.titleSpatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool
dc.typeJournal Article
rioxxterms.versionofrecord10.1098/rsif.2008.0249.focus
rioxxterms.licenseref.startdate2008-10-06
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfJOURNAL OF THE ROYAL SOCIETY INTERFACE
pubs.notesaffiliation: Neil, MAA (Reprint Author), Univ London Imperial Coll Sci Technol & Med, Dept Phys, Exhibit Rd, London SW7 2AZ, England. French, P. M. W.; Neil, M. A. A., Univ London Imperial Coll Sci Technol & Med, Dept Phys, London SW7 2AZ, England. Lanigan, Peter M. P.; Chan, Karen; Ninkovic, Tanya; Templer, Richard H.; French, P. M. W.; de Mello, A. J.; Parker, P. J.; Neil, M. A. A.; Ces, Oscar; Klug, D. R., Univ London Imperial Coll Sci Technol & Med, Chem Biol Ctr, Single Cell Prote Grp, London SW7 2AZ, England. Templer, Richard H.; de Mello, A. J.; Willison, K. R.; Ces, Oscar; Klug, D. R., Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England. Willison, K. R., Inst Canc Res, Chester Beatty Labs, London SW3 6JB, England. Parker, P. J., Canc Res UK, Lincolns Inn Fields, London WC2A 3PX, England. keywords: single-cell proteomics; optical trapping; plasma membrane; protein levels; membrane tether; emulsion keywords-plus: MEMBRANE CURVATURE; PLASMA-MEMBRANE; PROTEINS; LYSIS; TWEEZERS; DEFORMATION; EXTRACTION; BILAYER; ASSAYS research-areas: Science & Technology - Other Topics web-of-science-categories: Multidisciplinary Sciences author-email: [email protected] [email protected] researcherid-numbers: Neil, Mark/B-1184-2010 Parker, Peter/D-5192-2013 orcid-numbers: Neil, Mark/0000-0002-7720-8868 Templer, Richard/0000-0002-9911-8011 parker, peter/0000-0002-6218-2933 Chan, Karen/0000-0001-9947-4713 funding-acknowledgement: EPSRC [EP/C54269X/1, GR/S77721, EP/C541839/1]; Engineering and Physical Sciences Research Council [EP/C541839/1, EP/C54269X/1] funding-text: We thank the EPSRC for the award of grant EP/C54269X/1 and Platform grants GR/S77721 and EP/C541839/1. We would like to thank Dr Christopher Dunsby for establishing the optical platforms used during these experiments. number-of-cited-references: 36 times-cited: 11 usage-count-last-180-days: 1 usage-count-since-2013: 11 journal-iso: J. R. Soc. Interface doc-delivery-number: 342HS unique-id: ISI:000258775800007 oa: gold_or_bronze da: 2018-08-30
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation
pubs.volume5
pubs.embargo.termsNot known
icr.researchteamChromatin Regulationen_US
dc.contributor.icrauthorWillison, Keith


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