NetworKIN: a resource for exploring cellular phosphorylation networks
Date
2008-01ICR Author
Author
Linding, R
Jensen, LJ
Pasculescu, A
Olhovsky, M
Colwill, K
Bork, P
Yaffe, MB
Pawson, T
Type
Journal Article
Metadata
Show full item recordAbstract
Protein kinases control cellular responses by phosphorylating specific substrates. Recent proteome-wide mapping of protein phosphorylation sites by mass spectrometry has discovered thousands of in vivo sites. Systematically assigning all 518 human kinases to all these sites is a challenging problem. The NetworKIN database (http://networkin.info) integrates consensus substrate motifs with context modelling for improved prediction of cellular kinase-substrate relations. Based on the latest human phosphoproteome from the Phospho. ELM and PhosphoSite databases, the resource offers insight into phosphorylation-modulated interaction networks. Here, we describe how NetworKIN can be used for both global and targeted molecular studies. Via the web interface users can query the database of precomputed kinase-substrate relations or obtain predictions on novel phosphoproteins. The database currently contains a predicted phosphorylation network with 20 224 site-specific interactions involving 3978 phosphoproteins and 73 human kinases from 20 families.
Collections
Language
eng
License start date
2008-01
Citation
NUCLEIC ACIDS RESEARCH, 2008, 36 pp. D695 - D699
Publisher
OXFORD UNIV PRESS