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Visualizing the organization and reorganization of transcription complexes for gene expression

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Date
2008-08
ICR Author
Rappas, Mathieu
Author
Burrows, PC
Wigneshweraraj, S
Bose, D
Joly, N
Schumacher, J
Rappas, M
Pape, T
Stockley, PG
Zhang, X
Buck, M
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Type
Journal Article
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Abstract
Regulated gene expression requires control of the transcription machinery, frequently through the establishment of different functional states of the transcribing enzyme RNA polymerase and its attendant activator proteins. In bacteria, major adaptive responses use an enhancer-dependent RNA polymerase, activated for transcription by a class of ATPases that remodel initial promoter complexes to form transcriptionally proficient open promoter complexes. In the present article, we summarize the integrated use of site-specific protein cleavage and DNA cross-linking methods, as well as FRET (fluorescence resonance energy transfer) in combination with X-ray crystallography and cryo-electron microscopy to gain insight into the organization of the enhancer-dependent sigma(54)-RNA polymerase and the ATPase-driven activation mechanism.
URI
https://repository.icr.ac.uk/handle/internal/2443
DOI
https://doi.org/10.1042/BST0360776
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Language
eng
License start date
2008-08
Citation
BIOCHEMICAL SOCIETY TRANSACTIONS, 2008, 36 pp. 776 - 779
Publisher
PORTLAND PRESS LTD

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