Show simple item record

dc.contributor.authorBurrows, PC
dc.contributor.authorWigneshweraraj, S
dc.contributor.authorBose, D
dc.contributor.authorJoly, N
dc.contributor.authorSchumacher, J
dc.contributor.authorRappas, M
dc.contributor.authorPape, T
dc.contributor.authorStockley, PG
dc.contributor.authorZhang, X
dc.contributor.authorBuck, M
dc.identifier.citationBIOCHEMICAL SOCIETY TRANSACTIONS, 2008, 36 pp. 776 - 779
dc.description.abstractRegulated gene expression requires control of the transcription machinery, frequently through the establishment of different functional states of the transcribing enzyme RNA polymerase and its attendant activator proteins. In bacteria, major adaptive responses use an enhancer-dependent RNA polymerase, activated for transcription by a class of ATPases that remodel initial promoter complexes to form transcriptionally proficient open promoter complexes. In the present article, we summarize the integrated use of site-specific protein cleavage and DNA cross-linking methods, as well as FRET (fluorescence resonance energy transfer) in combination with X-ray crystallography and cryo-electron microscopy to gain insight into the organization of the enhancer-dependent sigma(54)-RNA polymerase and the ATPase-driven activation mechanism.
dc.format.extent776 - 779
dc.titleVisualizing the organization and reorganization of transcription complexes for gene expression
dc.typeJournal Article
rioxxterms.typeJournal Article/Review
pubs.notesaffiliation: Buck, M (Reprint Author), Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, Dept Life Sci, London SW7 2AZ, England. Burrows, Patricia C.; Joly, Nicolas; Schumacher, Joerg; Buck, Martin, Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, Dept Life Sci, London SW7 2AZ, England. Bose, Dan; Pape, Tilmann; Zhang, Xiaodong, Univ London Imperial Coll Sci Technol & Med, Div Biol, Dept Life Sci, London SW7 2AZ, England. Wigneshweraraj, Sivaramesh, Univ London Imperial Coll Sci Technol & Med, Dept Microbiol, Div Invest Sci, Fac Med, London SW7 2AZ, England. Wigneshweraraj, Sivaramesh, Univ London Imperial Coll Sci Technol & Med, Ctr Mol Microbiol & Infect, London SW7 2AZ, England. Rappas, Mathieu, Inst Canc Res, Sect Struct Biol, Chester Beatty Labs, London SW3 6JB, England. Stockley, Peter G., Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England. keywords: activator; ATPase; promoter DNA; RNA polymerase (RNAP); sigma(54); transcription keywords-plus: PROMOTER DNA COMPLEX; RNA-POLYMERASE; ALUMINUM FLUORIDE; BINDING; ACTIVATION; SIGMA-54; DOMAIN; HOLOENZYME; INITIATION; INSIGHTS research-areas: Biochemistry & Molecular Biology web-of-science-categories: Biochemistry & Molecular Biology author-email: [email protected] researcherid-numbers: Joly, Nicolas/H-6851-2018 orcid-numbers: Joly, Nicolas/0000-0003-2731-3827 Bose, Daniel/0000-0002-0276-6486 Pape, Tillmann/0000-0002-0612-3568 Zhang, Xiaodong/0000-0001-9786-7038 funding-acknowledgement: Biotechnology and Biological Sciences Research Council [BBS/B/03351]; Biotechnology and Biological Sciences Research Council [B19279] number-of-cited-references: 19 times-cited: 4 usage-count-last-180-days: 0 usage-count-since-2013: 3 journal-iso: Biochem. Soc. Trans. doc-delivery-number: 338QI unique-id: ISI:000258522200040 oa: gold_or_bronze da: 2018-08-30
pubs.notesNot known
pubs.embargo.termsNot known
dc.contributor.icrauthorRappas, Mathieuen

Files in this item


There are no files associated with this item.

This item appears in the following collection(s)

Show simple item record