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dc.contributor.authorFournier, F
dc.contributor.authorGardner, EM
dc.contributor.authorGuo, R
dc.contributor.authorDonaldson, PM
dc.contributor.authorBarter, LMC
dc.contributor.authorPalmer, DJ
dc.contributor.authorBarnett, CJ
dc.contributor.authorWillison, KR
dc.contributor.authorGould, IR
dc.contributor.authorKlug, DR
dc.date.accessioned2018-08-30T13:48:13Z
dc.date.issued2008-03-15
dc.identifier2
dc.identifier.citationANALYTICAL BIOCHEMISTRY, 2008, 374 pp. 358 - 365
dc.identifier.issn0003-2697
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2454
dc.identifier.doi10.1016/j.ab.2007.11.009
dc.description.abstractWe employ a particular form of two-dimensional infrared four-wave mixing (2DIR FWM) as a vibrational spectroscopic tool to quantify the amino acid content of a number of peptides. Vibrational features corresponding to ring modes of the aromatic groups of phenylalanine (Phe) and tyrosine (Tyr), as well as a methylene mode that is used as an internal reference, are identified. We show that the ratios of the integrated intensities, and the amplitudes, of the aromatic peaks of Phe and Tyr relative to the methylene integrated intensity, and amplitude, are proportional to the actual ratio of Phe and Tyr to CH2 in the samples within a precision of +/- 12.5%. This precision is shown to be sufficient to use this form of 2DIR spectroscopy as a possible proteins fingerprinting tool. (C) 2007 Elsevier Inc. All rights reserved.
dc.format.extent358 - 365
dc.languageeng
dc.language.isoeng
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.titleOptical fingerprinting of peptides using two-dimensional infrared spectroscopy: Proof of principle
dc.typeJournal Article
rioxxterms.versionofrecord10.1016/j.ab.2007.11.009
rioxxterms.licenseref.startdate2008-03-15
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfANALYTICAL BIOCHEMISTRY
pubs.notesaffiliation: Fournier, F (Reprint Author), Univ London Imperial Coll Sci Technol & Med, Dept Chem, Mol Dynam Res Grp, London SW7 2AZ, England. Fournier, Frederic; Gardner, Elizabeth M.; Guo, Rui; Donaldson, Paul M.; Barter, Laura M. C.; Barnett, Chris J.; Gould, Ian R.; Klug, David R., Univ London Imperial Coll Sci Technol & Med, Dept Chem, Mol Dynam Res Grp, London SW7 2AZ, England. Palmer, D. Jason, Univ Florence, European Lab Nonlinear Spectroscopy, I-50019 Florence, Italy. Willison, Keith R., Ctr Cellular & Mol Biol, Canc Res UK, Chester Beatty Labs, Inst Canc Res, London SW3 6JB, England. keywords: two-dimensional infrared spectroscopy; 2DIR; proteomics; peptides; phenylalanine; tyrosine; protein fingerprinting; amino acid keywords-plus: VIBRATIONAL SPECTROSCOPY; RAMAN-SPECTROSCOPY; 4-WAVE-MIXING SPECTROSCOPY; PROTEINS; DYNAMICS; SIGNATURES; SPECTRA; LIQUIDS research-areas: Biochemistry & Molecular Biology; Chemistry web-of-science-categories: Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical author-email: [email protected] researcherid-numbers: Klug, David/F-3357-2011 Guo, Rui/F-2384-2012 Donaldson, Paul/G-6462-2011 orcid-numbers: gould, ian/0000-0003-3559-0234 funding-acknowledgement: Engineering and Physical Sciences Research Council [EP/C530187/1] number-of-cited-references: 33 times-cited: 23 usage-count-last-180-days: 0 usage-count-since-2013: 12 journal-iso: Anal. Biochem. doc-delivery-number: 265EM unique-id: ISI:000253341900016 da: 2018-08-30
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation
pubs.volume374
pubs.embargo.termsNot known
icr.researchteamChromatin Regulationen_US
dc.contributor.icrauthorWillison, Keithen


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