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dc.contributor.authorSavva, R
dc.contributor.authorProdromou, C
dc.contributor.authorDriscoll, PC
dc.date.accessioned2018-08-30T15:03:09Z
dc.date.issued2007-11
dc.identifier21-22
dc.identifier.citationDRUG DISCOVERY TODAY, 2007, 12 pp. 939 - 947
dc.identifier.issn1359-6446
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2471
dc.identifier.doi10.1016/j.drudis.2007.08,016
dc.description.abstractIn this second of a two-part review encompassing random, combinatorial methods for soluble protein ‘domain hunting’, we focus upon the expression screening from DNA fragment libraries. Given a library of domain length-encoding DNA fragments assembled in expression vectors, it is necessary to devise reliable means to screen the sample DNA fragment population to find those that express stable, soluble target protein fragments, suitable for the required downstream aims. This review summarizes a variety of alternative strategies that have been employed to identify such stable truncates of full-length proteins. In addition, we review measures that can determine the quality of the expressed protein, the likely reliability of these measures, and the apparent extent of their application within the featured studies.
dc.format.extent939 - 947
dc.languageeng
dc.language.isoeng
dc.publisherELSEVIER SCI LTD
dc.titleDNA fragmentation based combinatorial approaches to soluble protein expression Part II: Library expression, screening and scale-up
dc.typeJournal Article
rioxxterms.versionofrecord10.1016/j.drudis.2007.08,016
rioxxterms.licenseref.startdate2007-11
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfDRUG DISCOVERY TODAY
pubs.notesaffiliation: Driscoll, PC (Reprint Author), UCL, Dept Biochem & Mol Biol, Inst Struct Mol Biol, Gower St, London WC1E 6BT, England. UCL, Dept Biochem & Mol Biol, Inst Struct Mol Biol, London WC1E 6BT, England. Univ London Birkbeck Coll, Sch Crystallog, Inst Struct Mol Biol, London WC1E 7HX, England. Inst Canc Res, Chester Beatty Labs, Sect Struct Biol, London SW3 6JB, England. Domainex Ltd, London Biosci Innovat Ctr, London NW1 0NH, England. keywords-plus: GREEN FLUORESCENT PROTEIN; TELOMERASE REVERSE-TRANSCRIPTASE; STRUCTURAL GENOMICS PIPELINE; THERMAL SHIFT ASSAYS; ESCHERICHIA-COLI; DIRECTED EVOLUTION; TERMINAL DOMAIN; DRUG DISCOVERY; IN-VIVO; STABILITY research-areas: Pharmacology & Pharmacy web-of-science-categories: Pharmacology & Pharmacy author-email: [email protected] researcherid-numbers: Driscoll, Paul/B-8007-2010 orcid-numbers: Driscoll, Paul/0000-0002-4124-6950 Prodromou, Chrisostomos/0000-0003-4320-1147 number-of-cited-references: 40 times-cited: 9 usage-count-last-180-days: 0 usage-count-since-2013: 8 journal-iso: Drug Discov. Today doc-delivery-number: 239AS unique-id: ISI:000251490600008 da: 2018-08-30
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.volume12
pubs.embargo.termsNot known
dc.contributor.icrauthorProdromou, Chrisostomosen


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