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dc.contributor.authorProdromou, C
dc.contributor.authorSawa, R
dc.contributor.authorDriscoll, PC
dc.identifier.citationDRUG DISCOVERY TODAY, 2007, 12 pp. 931 - 938
dc.description.abstractIn addressing a new drug discovery target, the generation of tractable protein substrates for functional and structural analyses can represent a significant hurdle. Traditional approaches rely on protein expression trials of multiple variants in various systems, frequently with limited success. The increasing knowledge base derived from genomics and structural proteomics initiatives assists the bioinformaticsled design of these experiments. Nevertheless, for many eukaryotic polypeptides, particularly those with relatively few homologues, the generation of useful protein products can still be a major challenge. This review describes the basis of efforts to forge an alternative ‘domain-hunting’ paradigm, based upon combinatorial sampling of expression construct libraries derived by fragmentation of the encoding DNA template, namely the methods and considerations in generating fragment length DNA from target genes. An accompanying review focuses upon the expression screening of such combinatorial DNA libraries for the sampling of the corresponding set of protein fragments.
dc.format.extent931 - 938
dc.publisherELSEVIER SCI LTD
dc.titleDNA fragmentation-based combinatorial approaches to soluble protein expression Part I. Generating DNA fragment libraries
dc.typeJournal Article
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfDRUG DISCOVERY TODAY
pubs.notesaffiliation: Driscoll, PC (Reprint Author), UCL, Dept Biochem & Mol Biol, Inst Struct Mol Biol, Gower St, London WC1E 6BT, England. UCL, Dept Biochem & Mol Biol, Inst Struct Mol Biol, London WC1E 6BT, England. Inst Canc Res, Chester Beatty Labs, Sect Struct Biol, London SW3 6JB, England. Univ London Birkbeck Coll, Sch Crystallog, Inst Struct Mol Biol, London WC1E 7HX, England. Domainex Ltd, London Biosci Innovat Ctr, London NW1 0NH, England. keywords-plus: ESCHERICHIA-COLI; RECOMBINANT PROTEINS; STRUCTURAL BIOLOGY; HUMAN GENOME; FUSION PROTEINS; LEAD DISCOVERY; 96-WELL FORMAT; DRUG DESIGN; DOMAINS; IDENTIFICATION research-areas: Pharmacology & Pharmacy web-of-science-categories: Pharmacology & Pharmacy author-email: [email protected] researcherid-numbers: Driscoll, Paul/B-8007-2010 orcid-numbers: Driscoll, Paul/0000-0002-4124-6950 Prodromou, Chrisostomos/0000-0003-4320-1147 number-of-cited-references: 53 times-cited: 17 usage-count-last-180-days: 0 usage-count-since-2013: 6 journal-iso: Drug Discov. Today doc-delivery-number: 239AS unique-id: ISI:000251490600007 da: 2018-08-30
pubs.notesNot known
pubs.embargo.termsNot known
dc.contributor.icrauthorProdromou, Chrisostomosen

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