Show simple item record

dc.contributor.authorAl-Buheissi, SZen_US
dc.contributor.authorCole, KJen_US
dc.contributor.authorHewer, Aen_US
dc.contributor.authorKumar, Ven_US
dc.contributor.authorBryan, RLen_US
dc.contributor.authorHudson, DLen_US
dc.contributor.authorPatel, HRen_US
dc.contributor.authorNathan, Sen_US
dc.contributor.authorMiller, RAen_US
dc.contributor.authorPhillips, DHen_US
dc.identifier.citationPROSTATE, 2006, 66 pp. 876 - 885en_US
dc.description.abstractBACKGROUND. Dietary heterocyclic amines (HCAs) are carcinogenic in rodent prostate requiring activation by enzymes such as cytochrome P450 (CYP) and N-acetyltransferase (NAT). METHODS. We investigated by Western blotting and immunohistochemistry the expression of CYP1A1, CYP1A2, and NAT1 in human prostate and in prostate epithelial cells (PECs) derived from primary cultures and tested their ability to activate the dietary carcinogen 2amino-3-methylimidazo[4,5-f]quinoline (IQ) and its N-hydroxy metabolite (N-OH-IQ) to DNA-damaging moieties. RESULTS. Western blotting identified CYP1A1, CYP1A2, and NAT1. Immunohistochemistry localized NAT1 to the cytoplasm of PECs. Inter-individual variation was observed in the expression levels of CYP1A1, 1A2, and NAT1 (11, 75, and 35-fold, respectively). PECs expressed CYP1A1 and NAT1 but not CYP1A2. When incubated with IQ or N-01-14Q, PECs formed DNA adducts indicating their ability to metabolically activate these compounds. CONCLUSIONS. Prostate cells possess the capacity to activate dietary carcinogens. PECs may provide a useful model system to study their role in prostate carcinogenesis.en_US
dc.format.extent876 - 885en_US
dc.titleThe expression of xenobiotic-metabolizing enzymes in human prostate and in prostate epithelial cells (PECs) derived from primary culturesen_US
dc.typeJournal Article
rioxxterms.typeJournal Article/Reviewen_US
pubs.notesaffiliation: Al-Buheissi, SZ (Reprint Author), Inst Canc Res, Sect Mol Carcinogenesis, Brookes Lawley Bldg, Cotswold Rd, Sutton SM2 5NG, Surrey, England. Inst Canc Res, Sect Mol Carcinogenesis, Brookes Lawley Bldg, Sutton SM2 5NG, Surrey, England. Whittington Hosp, Dept Urol, London N19 5NF, England. Whittington Hosp, Dept Histopathol, London N19 5NF, England. Inst Canc Res, Male Urol Canc Res Ctr, Prostate Stem Cell Lab, Surrey, England. UCL, Inst Urol, London, England. keywords: cytochrome P450; N-acetyltransferase; primary cultures; prostate cancer; prostate epithelial cells keywords-plus: ARYLAMINE N-ACETYLTRANSFERASE; TISSUE DISTRIBUTION; BREAST-CANCER; HUMAN CYTOCHROMES-P-450; PITUITARY FACTORS; CHOLERA-TOXIN; DNA-ADDUCTS; SERUM-FREE; IN-VITRO; CARCINOGEN research-areas: Endocrinology & Metabolism; Urology & Nephrology web-of-science-categories: Endocrinology & Metabolism; Urology & Nephrology author-email: [email protected] orcid-numbers: Phillips, David/0000-0001-8509-3485 number-of-cited-references: 51 times-cited: 7 usage-count-last-180-days: 0 usage-count-since-2013: 1 journal-iso: Prostate doc-delivery-number: 042RC unique-id: ISI:000237545600010 da: 2018-09-04en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Human Biomonitoring & Carcinogen Activation
pubs.embargo.termsNot knownen_US
icr.researchteamHuman Biomonitoring & Carcinogen Activationen_US
dc.contributor.icrauthorPhillips, David Hunteren_US
dc.contributor.icrauthorCole, Kathleenen_US

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record