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Multiplex-FISH (M-FISH): technique, developments and applications.

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Date
2006-01
ICR Author
Kearney, Lyndal Una
Author
Kearney, L
Type
Journal Article
Metadata
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Abstract
Multiplex FISH (M-FISH) represents one of the most significant developments in molecular cytogenetics of the past decade. Originally designed to generate 24 colour karyotyping, the technique has spawned many variations and an equally diverse range of applications. In tumour and leukaemia cytogenetics, the two groups that have been targeted represent both ends of the cytogenetic spectrum: those with an apparently normal karyotype (suspected of harbouring small rearrangements not detectable by conventional cytogenetics) and those with a complex aberrant karyotype (which are difficult to karyotype accurately due to the sheer number of aberrations). In research, mouse M-FISH provides a powerful tool to characterize mouse models of a disease. In addition, the ability to accurately karyotype single metaphases without selection makes M-FISH the perfect tool in chromosome breakage studies and for characterizing clonal evolution of tumours. Finally, M-FISH has emerged as the perfect partner for the developing genomic microarray (array CGH) technologies, providing a powerful approach to gene discovery.
URI
https://repository.icr.ac.uk/handle/internal/2538
DOI
https://doi.org/10.1159/000094202
Collections
  • Molecular Pathology
Subject
Humans
Burkitt Lymphoma
Chromosome Aberrations
Translocation, Genetic
In Situ Hybridization, Fluorescence
Karyotyping
Cytogenetics
Research team
Biology of Childhood Leukaemia
Language
eng
Date accepted
2006-04-06
License start date
2006-01
Citation
Cytogenetic and genome research, 2006, 114 (3-4), pp. 189 - 198

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