Comparative studies of intracellular Ca2+ in strongly and weakly metastatic rat prostate cancer cell lines

Abstract

The metastatic ability of prostate cancer cells involves differential expression of ionic mechanisms. In the present study, using electrophysiological recordings and intracellular Ca2+ measurements, we investigated Ca2+ related signalling in two rat prostate cancer (MAT-LyLu and AT-2) cell lines of markedly different metastatic potential. Whole-cell voltage clamp experiments indicated the absence of an inward current carried through voltage-dependent Ca2+ channels in either cell line. A Ca2+-dependent component was also absent in the voltage-activated outward K+ currents. Indo-1 microfluorimetry confirmed these results and also revealed marked differences in the resting level of intracellular Ca2+ and the ability of the two cell lines to regulate intracellular Ca2+. The C than the related but strongly metastatic MAT-weakly metastatic AT-2 cells displayed a significantly higher resting intracellular Ca2+ LyLu cell line. Increasing extracellular K+ decreased intracellular Ca2+ in the AT-2 but had no effect on intracellular Ca2+ levels in the MAT-LyLu cells. Furthermore, increasing extracellular Ca2+ increased intracellular Ca2+ in AT-2 but, again, had no effect on MAT-C permeation mechanism operating specifically LyLu cells. These results suggested the presence of a tonic, voltage-independent Ca2+ in the AT-2 cells. The influx of Ca2+ into the AT-2 cells was suppressed by both CdCl2) (100-300 mu M) and SKF-96365 (10-30 mu M). It is concluded that the strongly metastatic MAT-LyLu cell line lacks a voltage-independent basal Ca2+ influx mechanism that is present in the weakly metastatic AT-2 cells. (C) 2005 Elsevier Ltd. All rights reserved.