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dc.contributor.authorJonker, JWen_US
dc.contributor.authorFreeman, Jen_US
dc.contributor.authorBolscher, Een_US
dc.contributor.authorMusters, Sen_US
dc.contributor.authorAlvi, AJen_US
dc.contributor.authorTitley, Ien_US
dc.contributor.authorSchinkel, AHen_US
dc.contributor.authorDale, TCen_US
dc.identifier.citationSTEM CELLS, 2005, 23 pp. 1059 - 1065en_US
dc.description.abstractThe ability of cells to export Hoechst 33342 can be used to identify a subpopulation of cells (side population [SP]) with characteristics of stem cells in many tissues. The ATP-binding cassette transporters Bcrp1 (Abcg2) and Mdr1a/1b (Abcb1a/1b) have been implicated as being responsible for this phenotype. To further explore the involvement of these transporters in the SP phenotype, we have generated Bcrp1/Mdr1a/1b triple knockout mice and studied the effect of their absence on the SP in bone marrow and mammary gland. Whereas in bone marrow Bcrp1 was almost exclusively responsible for the SP, both transporters contributed to the SP phenotype in the mammary gland, where their combined absence resulted in a nearly complete loss of SP. Interestingly, bone marrow of Mdr1a/1b(-/-) mice frequently displayed an elevated SP, which was reversible by the Bcrp1 inhibitor Ko143, suggesting that Bcrp1 can compensate for the loss of Mdr1a/1b in bone marrow.en_US
dc.format.extent1059 - 1065en_US
dc.titleContribution of the ABC transporters Bcrp1 and Mdr1a/1b to the side population phenotype in mammary gland and bone marrow of miceen_US
dc.typeJournal Article
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfSTEM CELLSen_US
pubs.notesaffiliation: Schinkel, AH (Reprint Author), Netherlands Canc Inst, Div Expt Therapy, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands. Netherlands Canc Inst, Div Expt Therapy, NL-1066 CX Amsterdam, Netherlands. Cardiff Univ, Sch Biosci, Cardiff, S Glam, Wales. Inst Canc Res, Sect Cell & Mol Biol, London SW3 6JB, England. keywords: ATP-binding cassette transporter; adult bone marrow stem cells; progenitor cells; fluoresence-activated cell sorting analysis keywords-plus: BINDING CASSETTE TRANSPORTER; HEMATOPOIETIC STEM-CELLS; IN-VIVO; EXPRESSION; HOECHST-33342; MITOXANTRONE; BCRP1/ABCG2; VIABILITY; DRUGS; LINES research-areas: Cell Biology; Biotechnology & Applied Microbiology; Oncology; Hematology web-of-science-categories: Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Oncology; Cell Biology; Hematology author-email: researcherid-numbers: Dale, Trevor/D-3749-2009 orcid-numbers: Dale, Trevor/0000-0002-4880-9963 Jonker, Johan/0000-0002-3919-5437 number-of-cited-references: 33 times-cited: 101 usage-count-last-180-days: 0 usage-count-since-2013: 5 journal-iso: Stem Cells doc-delivery-number: 969EM unique-id: ISI:000232216200006 oa: gold_or_bronze da: 2018-09-06en_US
pubs.notesNot knownen_US
pubs.embargo.termsNot knownen_US
dc.contributor.icrauthorTitley, Ianen_US

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