Show simple item record

dc.contributor.authorAndersen, CLen_US
dc.contributor.authorGruszka-Westwood, Aen_US
dc.contributor.authorOstergaard, Men_US
dc.contributor.authorKoch, Jen_US
dc.contributor.authorJacobsen, Een_US
dc.contributor.authorKjeldsen, Een_US
dc.contributor.authorNielsen, Ben_US
dc.identifier.citationEUROPEAN JOURNAL OF HAEMATOLOGY, 2004, 72 pp. 390 - 402en_US
dc.description.abstractTo further characterise the genetic background of the two closely related B-lymphocytic malignancies hairy cell leukaemia (HCL), and splenic marginal zone lymphoma (SMZL) we have identified characteristic copy number imbalances by comparative genomic hybridisation (CGH). Based on these findings, areas of special interest were fine mapped, and relevant probes constructed for use in interphase-fluorescence in situ hybridisation (FISH) investigations. Thus, using the CGH data from 52 HCL and 61 SMZL patients, we identified the characteristic profiles of copy number imbalances for both diseases. These were a gain of 5q13-31 (19%) and loss of 7q22-q35 (6%) for HCL, and gain of 3q25 (28%), loss of 7q31 (16%), and gain of 12q15 (16%) for SMZL. A partial loss of 7q unsual for low-malignant B-cell diseases was found to be common to the two diseases. This loss was therefore fine mapped with BAC/PAC clones. Fine mapping revealed that in SMZL the minimal lost region covers 11.4 Mb spanning from 7q31.33 to 7q33 located between sequence tagged site (STS)-markers SHGC-3275 and D7S725. This area was distinct from the commonly deleted 7q region of myelodysplastic syndrome/acute myeloid leukaemia (MDS/AML). A FISH probe specific for the 7q region was constructed. Using this probe in an interphase-FISH investigation we showed the minimal lost 7q-region of HCL and SMZL to be one and the same. In one HCL case, this investigation furthermore showed the extent of the deleted region to be below the detection limit of CGH, whereas interphase-FISH screening of 36 chronic lymphocytic leukaemia (CLL) cases showed no deletion of the 7q area. In conclusion, we have identified characteristic profiles of copy number imbalances in HCL and SMZL and fine mapped the minimal extent of a commonly lost 7q area of special interest. We hypothesise that this region may contain (a) gene(s) important for the pathology of HCL and SMZL.en_US
dc.format.extent390 - 402en_US
dc.titleA narrow deletion of 7q is common to HCL, and SMZL, but not CLLen_US
dc.typeJournal Article
rioxxterms.typeJournal Article/Reviewen_US
pubs.notesaffiliation: Nielsen, B (Reprint Author), Aarhus Univ Hosp, Aarhus Kommune Hosp, Dept Hematol, Lab Canc Cytogenet, Tage Hansens Gade 2, DK-8000 Aarhus C, Denmark. Aarhus Univ Hosp, Aarhus Kommune Hosp, Dept Hematol, Lab Canc Cytogenet, DK-8000 Aarhus C, Denmark. Royal Marsden NHS Trust, Inst Canc Res, Acad Dept Haematol & Cytogenet, London, England. Aarhus Univ Hosp, Aarhus Kommune Hosp, Lab Immunohematol, Aarhus C, Denmark. keywords: hairy cell leukaemia; splenic marginal zone lymphoma; chronic lymphocytic leukaemia; comparative genomic hybridization; 7q loss keywords-plus: HAIRY-CELL LEUKEMIA; COMPARATIVE GENOMIC HYBRIDIZATION; MOLECULAR CYTOGENETIC ANALYSIS; CHRONIC LYMPHOCYTIC-LEUKEMIA; MARGINAL ZONE LYMPHOMA; TUMOR-SUPPRESSOR GENE; VILLOUS LYMPHOCYTES; SPLENIC LYMPHOMA; CHROMOSOMAL GAINS; IMBALANCES research-areas: Hematology web-of-science-categories: Hematology author-email: [email protected] researcherid-numbers: Andersen, Claus Lindbjerg/A-9217-2012 Andersen, Claus/C-1477-2017 orcid-numbers: Andersen, Claus Lindbjerg/0000-0002-7406-2103 Andersen, Claus/0000-0002-7406-2103 Gruszka, Alicja M/0000-0002-5359-0281 number-of-cited-references: 32 times-cited: 24 usage-count-last-180-days: 0 usage-count-since-2013: 0 journal-iso: Eur. J. Haematol. doc-delivery-number: 818FG unique-id: ISI:000221230400002 da: 2018-09-10en_US
pubs.notesNot knownen_US
pubs.embargo.termsNot knownen_US
dc.contributor.icrauthorGruszka-Westwood, Alicjaen_US

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record