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dc.contributor.authorSonoki, T
dc.contributor.authorWillis, TG
dc.contributor.authorOscier, DG
dc.contributor.authorKarran, EL
dc.contributor.authorSiebert, R
dc.contributor.authorDyer, MJS
dc.identifier.citationLEUKEMIA, 2004, 18 pp. 2026 - 2031
dc.description.abstractMolecular cloning of immunoglobulin heavy chain (IGH) translocation breakpoints identifies genes of biological importance in the development of normal and malignant B cells. Long-distance inverse PCR (LDI-PCR) was first applied to amplification of IGH gene translocations targeted to the joining (IGHJ) regions. We report here successful amplification of the breakpoint of IGH translocations targeted to switch (IGHS) regions by LDI-PCR. To detect IGHS translocations, Southern blot assays using 50 and 30 switch probes were performed. Illegitimate Smu rearrangements were amplified from the 50 end (5’Smu LDI-PCR) from the alternative derivative chromosome, and those of Sgamma or Salpha were amplified from the 3’ end (3’Sgamma or 3’alpha LDI-PCR) from the derivative chromosome 14. Using a combination of these methods, we have succeeded in amplifying IGHS translocation breakpoints involving FGFR3/MMSET on 4p16, BCL6 on 3q27, MYC on 8q24, IRTA1 on 1q21 and PAX5 on 9p13 as well as BCL11A on 2p13 and CCND3 on 6p21. The combination of LDI-PCR for IGHJ and IGHS allows rapid molecular cloning of almost all IGH gene translocation breakpoints.
dc.format.extent2026 - 2031
dc.titleRapid amplification of immunoglobulin heavy chain switch (IGHS) translocation breakpoints using long-distance inverse PCR
dc.typeJournal Article
rioxxterms.typeJournal Article/Review
pubs.notesaffiliation: Dyer, MJS (Reprint Author), Univ Leicester, MRC, Toxicol Unit, Hodgkin Bldg,POB 138,Lancaster Rd, Leicester LE1 9HN, Leics, England. Univ Leicester, MRC, Toxicol Unit, Leicester LE1 9HN, Leics, England. Inst Canc Res, London SW3 6JB, England. Royal Bournemouth Hosp, Dept Haematol, Bournemouth, Dorset, England. Univ Hosp Schleswig Holstein, Inst Human Genet, Kiel, Germany. Kumamoto Univ, Sch Med, Kumamoto 860, Japan. keywords: IGH switch region; translocation; LDI-PCR keywords-plus: NON-HODGKINS-LYMPHOMA; MULTIPLE-MYELOMA; CHROMOSOMAL TRANSLOCATIONS; CELL-LINE; B-CELLS; GENE; EXPRESSION; LOCUS; ESTABLISHMENT; TRANSCRIPTION research-areas: Oncology; Hematology web-of-science-categories: Oncology; Hematology author-email: [email protected] researcherid-numbers: Siebert, Reiner/A-8049-2010 orcid-numbers: Dyer, Martin/0000-0002-5033-2236 number-of-cited-references: 26 times-cited: 20 usage-count-last-180-days: 0 usage-count-since-2013: 2 journal-iso: Leukemia doc-delivery-number: 872ZV unique-id: ISI:000225249100015 oa: gold_or_bronze da: 2018-09-10
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