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dc.contributor.authorPhillips, DH
dc.contributor.authorHewer, A
dc.contributor.authorScholefield, JH
dc.contributor.authorSkinner, P
dc.date.accessioned2018-09-11T10:16:51Z
dc.date.issued2004
dc.identifier2
dc.identifier.citationMutation Research/Genetic Toxicology and Environmental Mutagenesis, 2004, 560 pp. 167 - 172
dc.identifier.issn1383-5718
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2622
dc.identifier.doi10.1016/j.mrgentox.2004.02.014
dc.description.abstractSeveral studies have identified tobacco smoking as a risk factor for anal cancer in both women and men. Samples of anal epithelium from haemorrhoidectomy specimens from current smokers (n=20) and age-matched life-long non-smokers (n=16) were analysed for DNA adducts by the nuclease P1 digestion enhancement procedure of 32P-postlabelling analysis. The study included 14 men and 22 women. Both qualitative and quantitative differences in the adduct profiles were observed between the smokers and non-smokers. The mean adduct level was significantly higher in the smokers than in the non-smokers (1.88±0.71 (S.D.) versus 1.36±0.60 adducts per 108 nucleotides, P=0.02, two-tailed unpaired t-test with Welch’s correction); furthermore, the adduct pattern seen in two-dimensional chromatograms revealed the smoking-related diagonal radioactive zone in 17/20 smokers, but not in any of the non-smokers (P<0.00001, Fisher’s exact test). These results indicate that components of tobacco smoke inflict genotoxic damage in the anal epithelium of smokers and provide a plausible mechanism for a causal association between smoking and anal cancer.
dc.format.extent167 - 172
dc.languageeng
dc.language.isoeng
dc.titleSmoking-related DNA adducts in anal epithelium
dc.typeJournal Article
rioxxterms.versionofrecord10.1016/j.mrgentox.2004.02.014
rioxxterms.licenseref.startdate2004
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfMutation Research/Genetic Toxicology and Environmental Mutagenesis
pubs.noteskeywords: DNA adducts, -postlabelling, Anal cancer, Tobacco smoking
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Human Biomonitoring & Carcinogen Activation
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Human Biomonitoring & Carcinogen Activation
pubs.volume560
pubs.embargo.termsNot known
icr.researchteamHuman Biomonitoring & Carcinogen Activationen_US
dc.contributor.icrauthorPhillips, David Hunteren
dc.contributor.icrauthorHewer, Alanen


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