Publications Repository

Publications Repository

View Item 
  •   Home
  • ICR Divisions
  • Closed Research Teams
  • View Item
  • Home
  • ICR Divisions
  • Closed Research Teams
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Role of estrogen receptor in regulation of polycyclic aromatic hydrocarbon metabolic activation in lung

Thumbnail
Publication Date
2004
ICR Author
Phillips, David Hunter
Hewer, Alan
Author
Berge, G
Mollerup, S
Øvrebø, S
Hewer, A
Phillips, DH
Eilertsen, E
Haugen, A
Type
Journal Article
Metadata
Show full item record
Abstract
Epidemiological and biochemical studies have indicated that females may be at greater risk of smoking associated lung cancer compared with males. Among lung cancer patients, female smokers have been found to have higher levels of PAH-related DNA adducts and CYP1A1 gene expression in their normal lung tissue compared to male smokers. A possible role of steroid hormones in these sex differences via interactions between aryl hydrocarbon receptor and estrogen receptor mediated cellular effects has been suggested. In the present study the impact of the estrogen receptor (ERα) on CYP1A1 and CYP1B1 gene expression was studied in vitro in human bronchial epithelial cells. Transient transfection of the BEP2D cell line with ERα influenced neither constitutive expression of CYP1A1 or CYP1B1 nor induction of these genes by TCDD as measured by real-time RT-PCR. ERα had no effect on the constitutive or TCDD-induced enzymatic activity of CYP1A1 (EROD). We also studied the effect of steroid hormones on lung PAH metabolic activation in A/J mice. Intact and ovariectomized female mice were orally exposed to a single dose of benzo[a]pyrene. Ovariectomy did not influence the levels of either benzo[a]pyrene-derived protein or DNA adducts in the lung tissue measured by HPLC and 32P-postlabeling, respectively. In conclusion, the present data do not support the hypothesis of a role of estrogen or the ERα in regulating the metabolic activation of polycyclic aromatic hydrocarbons in lung.
URL
https://repository.icr.ac.uk/handle/internal/2632
Collections
  • Closed Research Teams
Version of record
10.1016/j.lungcan.2004.02.014
Research team
Human Biomonitoring & Carcinogen Activation
License start date
2004
Citation
Lung Cancer, 2004, 45 pp. 289 - 297

Browse

All of ICR repositoryICR DivisionsIssue dateAuthorsTitlesSubjectsThis collectionIssue dateAuthorsTitlesSubjects

Statistics

Most popular itemsStatistics by countryMost popular authors
  • Login
  • Registered office: The Institute of Cancer Research, 123 Old Brompton Road, London, SW7 3RP
    A Charity, Not for Profit. Company Limited by Guarantee.
    Registered in England No. 534147. VAT Registration No. GB 849 0581 02.