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dc.contributor.authorBaumann, M
dc.contributor.authorMamais, A
dc.contributor.authorMcBlane, F
dc.contributor.authorXiao, H
dc.contributor.authorBoyes, J
dc.date.accessioned2018-09-12T11:45:38Z
dc.date.issued2003-10-01
dc.identifier19
dc.identifier.citationEMBO JOURNAL, 2003, 22 pp. 5197 - 5207
dc.identifier.issn0261-4189
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2654
dc.identifier.doi10.1093/emboj/cdg487
dc.description.abstractA key component in the regulation of V(D)J recombination is control of the accessibility of RAG proteins to recombination signal sequences (RSS). Nucleosomes are known to inhibit this accessibility. We show here that the signal sequence itself represses accessibility by causing nucleosome positioning over the RSS. This positioning is mediated, in vitro and in vivo, by the conserved nonamer of the RSS. Consistent with this strong positioning, nucleosomes at RSSs are resistant to remodelling by nucleosome sliding. In vivo we find that consensus RSSs are preferentially protected, whereas those that lack a consensus nonamer, including some cryptic RSSs, fail to position nucleosomes. Decreased protection of these non-consensus RSSs correlates with their increased use in recombination assays. We therefore suggest that nucleosome positioning by RSSs provides a previously unanticipated level of protection and regulation of V(D)J recombination.
dc.format.extent5197 - 5207
dc.languageeng
dc.language.isoeng
dc.publisherOXFORD UNIV PRESS
dc.titleRegulation of V(D)J recombination by nucleosome positioning at recombination signal sequences
dc.typeJournal Article
rioxxterms.versionofrecord10.1093/emboj/cdg487
rioxxterms.licenseref.startdate2003-10-01
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfEMBO JOURNAL
pubs.notesaffiliation: Boyes, J (Reprint Author), Inst Canc Res, 237 Fulham Rd, London SW3 6JB, England. Inst Canc Res, London SW3 6JB, England. European Inst Oncol, I-20141 Milan, Italy. NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA. keywords: chromatin remodelling; chromosomal translocation; nucleosome positioning; RAGs; V(D)J recombination keywords-plus: HISTONE ACETYLATION; CHROMATIN STRUCTURE; IN-VITRO; DNA; PROMOTER; CLEAVAGE; GENE; TRANSCRIPTION; RECEPTOR; ACCESSIBILITY research-areas: Biochemistry & Molecular Biology; Cell Biology web-of-science-categories: Biochemistry & Molecular Biology; Cell Biology number-of-cited-references: 46 times-cited: 61 usage-count-last-180-days: 0 usage-count-since-2013: 2 journal-iso: Embo J. doc-delivery-number: 728RZ unique-id: ISI:000185731300030 oa: gold_or_bronze da: 2018-09-11
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Molecular Embryology
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Molecular Embryology
pubs.volume22
pubs.embargo.termsNot known
icr.researchteamMolecular Embryologyen_US
dc.contributor.icrauthorBoyes, Joanen
dc.contributor.icrauthorMamais, Adamantiosen
dc.contributor.icrauthorBaumann, Matthiasen


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