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dc.contributor.authorHoffmann, R
dc.contributor.authorSeidl, T
dc.contributor.authorBruno, L
dc.contributor.authorDugas, M
dc.date.accessioned2018-09-12T11:45:48Z
dc.date.issued2003-10
dc.identifier4
dc.identifier.citationJOURNAL OF LEUKOCYTE BIOLOGY, 2003, 74 pp. 602 - 610
dc.identifier.issn0741-5400
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2655
dc.identifier.doi10.1189/jlb.0203085
dc.description.abstractB and T lymphocytes develop through a series of cellular stages, which are defined by recombination status of the immunoglobulin and T cell receptor loci and can be separated by analysis of cell-surface markers. We evaluated how well 26 and 41 samples from five and eight developmental stages of B and T cell development, respectively, could be correctly assigned to their lineage of origin and developmental stage by analysis of the expression of 13,026 genes and expressed sequence tags (ESTs). The RNA expression patterns of eight genes correctly classified all 67 samples as belonging to the B cell or to the T cell lineage. Ninety-two to 100% of B-lineage samples could be correctly assigned to the protein-defined developmental stage by the RNA expression pattern of 29 genes. By contrast, RNA expression patterns of 39 genes were necessary to correctly assign 85-100% of T-lineage samples to the correct developmental stage. The sets of genes used for these classifications contain ESTs as well as known genes that have not previously been associated with lymphocyte development. Graphical display of the classifications shows that B-lineage samples are well separated from T-lineage samples, and samples from the five stages of B cell development are well separated from each other. By contrast, samples from the eight stages of T cell development cannot be separated precisely. We conclude that the protein markers currently widely used for separating stages of B cell development better identify molecularly distinct stages than those used for separating stages of T cell development.
dc.format.extent602 - 610
dc.languageeng
dc.language.isoeng
dc.publisherFEDERATION AMER SOC EXP BIOL
dc.titleDevelopmental markers of B cells are superior to those of T cells for identification of stages with distinct gene expression profiles
dc.typeJournal Article
rioxxterms.versionofrecord10.1189/jlb.0203085
rioxxterms.licenseref.startdate2003-10
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfJOURNAL OF LEUKOCYTE BIOLOGY
pubs.notesaffiliation: Hoffmann, R (Reprint Author), Max Von Pettenkofer Inst, Dept Bacteriol, Pettenkoferstr 9A, D-80336 Munich, Germany. Max Von Pettenkofer Inst, Dept Bacteriol, D-80336 Munich, Germany. Inst Canc Res, Chester Beatty Labs, Sect Gene Funct & Regulat, London SW3 6JB, England. Univ Munich, Dept Med Informat Biometr & Epidemiol, Munich, Germany. keywords: microarray; B/T cell lineage; principal component analysis keywords-plus: OLIGONUCLEOTIDE ARRAYS; RECEPTOR; PREDICTION; PROTEIN; MICE; CLASSIFICATION; ACTIVATION; CANCER; FAMILY; MURINE research-areas: Cell Biology; Hematology; Immunology web-of-science-categories: Cell Biology; Hematology; Immunology researcherid-numbers: Hoffmann, Reinhard/I-8748-2018 Seidl, Thomas/B-5713-2014 Dugas, Martin/F-5454-2011 orcid-numbers: Hoffmann, Reinhard/0000-0003-1514-1183 Seidl, Thomas/0000-0003-0889-7054 number-of-cited-references: 38 times-cited: 3 usage-count-last-180-days: 0 usage-count-since-2013: 0 journal-iso: J. Leukoc. Biol. doc-delivery-number: 755FF unique-id: ISI:000187392300016 da: 2018-09-11
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.volume74
pubs.embargo.termsNot known
dc.contributor.icrauthorLudovica, Brunoen


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