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dc.contributor.authorTelli-Karakoç, Fen_US
dc.contributor.authorRuddock, PJen_US
dc.contributor.authorBird, DJen_US
dc.contributor.authorHewer, Aen_US
dc.contributor.authorSchanke, AVen_US
dc.contributor.authorPhillips, DHen_US
dc.contributor.authorPeters, LDen_US
dc.identifier.citationMarine Environmental Research, 2002, 54 pp. 511 - 515en_US
dc.description.abstractJuvenile turbot (Scophthalmus maximus) were injected intraperitonteally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled 1 and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/108 nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P <0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 3OH BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/108 nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite–DNA interactions occurs indicative of other oxidative processes.en_US
dc.format.extent511 - 515en_US
dc.titleCorrelative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyreneen_US
dc.typeJournal Article
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfMarine Environmental Researchen_US
pubs.noteskeywords: 7-Ethoxyresorufin -deethylase activity, Benzo[a]pyrene, Bile, Metabolites, DNA adduct, Postlabellingen_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Human Biomonitoring & Carcinogen Activation
pubs.embargo.termsNot knownen_US
icr.researchteamHuman Biomonitoring & Carcinogen Activationen_US
dc.contributor.icrauthorPhillips, David Hunteren_US

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