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dc.contributor.authorParkinson, GN
dc.contributor.authorLee, MPH
dc.contributor.authorNeidle, S
dc.identifier.citationNATURE, 2002, 417 pp. 876 - 880
dc.description.abstractTelomeric ends of chromosomes, which comprise noncoding repeat sequences of guanine-rich DNA, are fundamental in protecting the cell from recombination and degradation(1). Disruption of telomere maintenance leads to eventual cell death, which can be exploited for therapeutic intervention in cancer. Telomeric DNA sequences can form four-stranded (quadruplex) structures(2-4), which may be involved in the structure of telomere ends(5). Here we describe the crystal structure of a quadruplex formed from four consecutive human telomeric DNA repeats and grown at a K+ concentration that approximates its intracellular concentration. K+ ions are observed in the structure. The folding and appearance of the DNA in this intramolecular quadruplex is fundamentally different from the published Na+-containing quadruplex structures(2,4,6). All four DNA strands are parallel, with the three linking trinucleotide loops positioned on the exterior of the quadruplex core, in a propeller-like arrangement. The adenine in each TTA linking trinucleotide loop is swung back so that it intercalates between the two thymines. This DNA structure suggests a straightforward path for telomere folding and unfolding, as well as ways in which it can recognize telomere-associated proteins.
dc.format.extent876 - 880
dc.titleCrystal structure of parallel quadruplexes from human telomeric DNA
dc.typeJournal Article
rioxxterms.typeJournal Article/Review
pubs.notesaffiliation: Neidle, S (Reprint Author), Inst Canc Res, Chester Beatty Labs, Canc Res UK Biomol Struct Unit, 237 Fulham Rd, London SW3 6JB, England. Inst Canc Res, Chester Beatty Labs, Canc Res UK Biomol Struct Unit, London SW3 6JB, England. keywords-plus: ANGSTROM RESOLUTION; G-QUARTETS; OLIGONUCLEOTIDES; DERIVATIVES; MECHANISMS; INHIBITION; TETRAPLEX; COMPLEX; TARGET; DESIGN research-areas: Science & Technology - Other Topics web-of-science-categories: Multidisciplinary Sciences number-of-cited-references: 29 times-cited: 1335 usage-count-last-180-days: 19 usage-count-since-2013: 227 journal-iso: Nature doc-delivery-number: 563YM unique-id: ISI:000176285600053 da: 2018-09-17
pubs.notesNot known
pubs.embargo.termsNot known
dc.contributor.icrauthorNeidle, Stephen

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