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Mutagenic fingerprint of ozone in human cells

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Date
2002
ICR Author
Phillips, David Hunter
Osborne, Martin
Author
Jorge, SAC
Menck, CFM
Sies, H
Osborne, MR
Phillips, DH
Sarasin, A
Stary, A
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Type
Journal Article
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Abstract
Ozone is an important factor in urban pollution and represents a major concern for human health. The chemical reactivity of ozone toward biological targets and particularly its genotoxicity supports a possible link between exposure and cancer risk, but no molecular data exist on its mutagenic potential in human cells. Using a shuttle vector, we showed that ozone is indeed a potent mutagen and we characterized the mutation spectrum it produced in human cells. Almost all mutations are base substitutions, essentially located at G:Cs (75%), typical of reactive oxygen species (ROS), but occurring in a specific pattern, i.e. a similar extent of GC:TA (28%), GC:CG (23%) and GC:AT (23%). The targeted distribution of mutations and identification of hotspot sequences define the first molecular fingerprint of mutations induced by ozone in human cells. Possible applications derived from our results with respect to ozone genotoxicity should help determining quantifiable biomarkers of ozone exposure in human health, especially for carcinogenesis.
URI
https://repository.icr.ac.uk/handle/internal/2774
DOI
https://doi.org/10.1016/S1568-7864(02)00011-3
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Research team
Human Biomonitoring & Carcinogen Activation
Language
eng
License start date
2002
Citation
DNA Repair, 2002, 1 pp. 369 - 378

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