Show simple item record

dc.contributor.authorGao, YN
dc.contributor.authorLu, YJ
dc.contributor.authorXue, SA
dc.contributor.authorChen, HL
dc.contributor.authorWedderburn, N
dc.contributor.authorGriffin, BE
dc.date.accessioned2018-09-18T14:37:45Z
dc.date.issued2002-01-24
dc.identifier5
dc.identifier.citationONCOGENE, 2002, 21 pp. 825 - 835
dc.identifier.issn0950-9232
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2777
dc.identifier.doi10.1038/sj/onc/1205130
dc.description.abstractTransfection of primate tissue explants with a specific sub-fragment (p31) of EBV DNA results in epithelial (but no other) cells proliferating indefinitely (becoming ‘immortalized’) without evidence of a ‘growth crisis’. Molecular evidence supports integration of viral information into the host chromosome, and an early genotypic alteration involving specific amplification of a subcomponent (IR1) of p31 DNA, followed by apparent loss of viral DNA from chromosomes, consistent with a ‘hit and run’ mechanism. However, analysis at the individual cell level during long-term culture, by FISH techniques, reveals chromosomal alferations, and viral sequences surviving within double minute (DM) bodies. Changing growth patterns occurring at different stages during propagation (>a year in culture) may be explained by sporadic reintegration of surviving viral DNA into the host chromosome. Notably, throughout culture, telomere lengths in chromosomal DNAs do not alter but rather retain the length observed in the primary cell populations. Introduction of a growth stimulating function of EBV, BARF1, into the immortalized, nonclonable epithelial cells under conditions which permit overexpression, allows clonal populations to be derived. Based on the data, mechanisms of immortalization, in the absence of a proven viral oncogene in p31 DNA, and possible genes involved, are considered.
dc.format.extent825 - 835
dc.languageeng
dc.language.isoeng
dc.titleHypothesis: a novel route for immortalization of epithelial cells by Epstein-Barr virus
dc.typeJournal Article
rioxxterms.versionofrecord10.1038/sj/onc/1205130
rioxxterms.licenseref.startdate2002-01-24
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfONCOGENE
pubs.notesresearcherid-numbers: Xue, Shao-An/A-2735-2011 orcid-numbers: Lu, Yong-Jie/0000-0001-6174-6621 unique-id: ISI:000173427000013
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.volume21
pubs.embargo.termsNot known
dc.contributor.icrauthorLu, Yong-Jieen


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following collection(s)

Show simple item record