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dc.contributor.authorGao, YNen_US
dc.contributor.authorLu, YJen_US
dc.contributor.authorXue, SAen_US
dc.contributor.authorChen, HLen_US
dc.contributor.authorWedderburn, Nen_US
dc.contributor.authorGriffin, BEen_US
dc.identifier.citationONCOGENE, 2002, 21 pp. 825 - 835en_US
dc.description.abstractTransfection of primate tissue explants with a specific sub-fragment (p31) of EBV DNA results in epithelial (but no other) cells proliferating indefinitely (becoming ‘immortalized’) without evidence of a ‘growth crisis’. Molecular evidence supports integration of viral information into the host chromosome, and an early genotypic alteration involving specific amplification of a subcomponent (IR1) of p31 DNA, followed by apparent loss of viral DNA from chromosomes, consistent with a ‘hit and run’ mechanism. However, analysis at the individual cell level during long-term culture, by FISH techniques, reveals chromosomal alferations, and viral sequences surviving within double minute (DM) bodies. Changing growth patterns occurring at different stages during propagation (>a year in culture) may be explained by sporadic reintegration of surviving viral DNA into the host chromosome. Notably, throughout culture, telomere lengths in chromosomal DNAs do not alter but rather retain the length observed in the primary cell populations. Introduction of a growth stimulating function of EBV, BARF1, into the immortalized, nonclonable epithelial cells under conditions which permit overexpression, allows clonal populations to be derived. Based on the data, mechanisms of immortalization, in the absence of a proven viral oncogene in p31 DNA, and possible genes involved, are considered.en_US
dc.format.extent825 - 835en_US
dc.titleHypothesis: a novel route for immortalization of epithelial cells by Epstein-Barr virusen_US
dc.typeJournal Article
rioxxterms.typeJournal Article/Reviewen_US
pubs.notesresearcherid-numbers: Xue, Shao-An/A-2735-2011 orcid-numbers: Lu, Yong-Jie/0000-0001-6174-6621 unique-id: ISI:000173427000013en_US
pubs.notesNot knownen_US
pubs.embargo.termsNot knownen_US
dc.contributor.icrauthorLu, Yong-Jieen_US

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