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Phosphorylation of HDM2 by Akt

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Date
2002-03-21
ICR Author
Ashcroft, Margaret
Author
Ashcroft, M
Ludwig, RL
Woods, DB
Copeland, TD
Weber, HO
MacRae, EJ
Vousden, KH
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Type
Journal Article
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Abstract
The HDM2 protein is a key regulator of the tumour suppressor, p53. Control of HDM2 function is critical for normal cell proliferation and stress responses, and it is becoming evident that multiple modifications of HDM2 can regulate its function within cells. In this study we show that HDM2 associated with the serinethreonine kinase, Akt, in response to growth factor stimulation of human primary cells. This association was concurrent with phosphorylation of Akt (at Ser 473), and resulted in elevated expression of HDM2 and enhanced nuclear localization. However, analysis of HDM2 proteins mutated at the consensus Akt recognition sites at serines 166 and 186 indicated that modification at these residues was not sufficient for the increased expression of the protein, which was blocked by the PI3 kinase inhibitor LY294002. Tryptic peptide and mutational analyses revealed evidence for an Akt phosphorylation site in HDM2 additional to the two consensus sites.
URI
https://repository.icr.ac.uk/handle/internal/2781
DOI
https://doi.org/10.1038/sj/onc/1205276
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  • Other ICR Research
Language
eng
License start date
2002-03-21
Citation
ONCOGENE, 2002, 21 pp. 1955 - 1962

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