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Comparative genomic hybridization and amplotyping by arbitrarily primed PCR in stage A B-CLL

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Date
2001-10-01
ICR Author
Matutes, Estella
Author
Odero, MD
Soto, JL
Matutes, E
Martin-Subero, JI
Zudaire, I
Rao, PH
Cigudosa, JC
Ardanaz, MT
Chaganti, RSK
Perucho, M
Calasanz, MJ
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Type
Journal Article
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Abstract
Cytogenetic analysis is useful in the diagnosis and to assess prognosis of B-cell chronic lymphocytic leukemia (B-CLL). However, successful cytogenetics by standard techniques has been hindered by the low in vitro mitotic activity of the malignant B-cell population. Fluorescence in situ hybridization (FISH) has become a useful tool, but it does not provide an overall view of the aberrations. To overcome this hurdle, two DNA-based techniques have been tested in the present study: comparative genomic hybridization (CGH) and amplotyping by arbitrarily primed PCR (AP-PCR). Comparative genomic hybridization resolution depends upon the 400-bands of the human standard karyotype. AP-PCR allows detection of allelic losses and gains in tumor cells by PCR fingerprinting, thus its resolution is at the molecular level. Both techniques were performed in 23 patients with stage A B-CLL at diagnosis. The results were compared with FISH. The sensitivity of AP-PCR was greater than CGH (62% vs 43%). The use of CGH combined with AP-PCR allowed to detect genetic abnormalities in 79% (15/19) of patients in whom G-banding wa,, not informative. providing a global view of the aberrations in a sole experiment. This study shows that combining these two methods with FISH, makes possible a more precise genetic characterization of patients with B-CLL. (C) 2001 Elsevier Science Inc. All rights reserved.
URI
https://repository.icr.ac.uk/handle/internal/2821
DOI
https://doi.org/10.1016/S0165-4608(01)00470-8
Collections
  • Clinical Studies
  • Molecular Pathology
Research team
Molecular Haematology (including Cytogenetics Group and Cell Markers)
Language
eng
License start date
2001-10-01
Citation
CANCER GENETICS AND CYTOGENETICS, 2001, 130 pp. 8 - 13

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