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dc.contributor.authorOdero, MDen_US
dc.contributor.authorSoto, JLen_US
dc.contributor.authorMatutes, Een_US
dc.contributor.authorMartin-Subero, JIen_US
dc.contributor.authorZudaire, Ien_US
dc.contributor.authorRao, PHen_US
dc.contributor.authorCigudosa, JCen_US
dc.contributor.authorArdanaz, MTen_US
dc.contributor.authorChaganti, RSKen_US
dc.contributor.authorPerucho, Men_US
dc.contributor.authorCalasanz, MJen_US
dc.date.accessioned2018-09-24T15:50:18Z
dc.date.issued2001-10-01en_US
dc.identifier1en_US
dc.identifier.citationCANCER GENETICS AND CYTOGENETICS, 2001, 130 pp. 8 - 13en_US
dc.identifier.issn0165-4608en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2821
dc.identifier.doi10.1016/S0165-4608(01)00470-8en_US
dc.description.abstractCytogenetic analysis is useful in the diagnosis and to assess prognosis of B-cell chronic lymphocytic leukemia (B-CLL). However, successful cytogenetics by standard techniques has been hindered by the low in vitro mitotic activity of the malignant B-cell population. Fluorescence in situ hybridization (FISH) has become a useful tool, but it does not provide an overall view of the aberrations. To overcome this hurdle, two DNA-based techniques have been tested in the present study: comparative genomic hybridization (CGH) and amplotyping by arbitrarily primed PCR (AP-PCR). Comparative genomic hybridization resolution depends upon the 400-bands of the human standard karyotype. AP-PCR allows detection of allelic losses and gains in tumor cells by PCR fingerprinting, thus its resolution is at the molecular level. Both techniques were performed in 23 patients with stage A B-CLL at diagnosis. The results were compared with FISH. The sensitivity of AP-PCR was greater than CGH (62% vs 43%). The use of CGH combined with AP-PCR allowed to detect genetic abnormalities in 79% (15/19) of patients in whom G-banding wa,, not informative. providing a global view of the aberrations in a sole experiment. This study shows that combining these two methods with FISH, makes possible a more precise genetic characterization of patients with B-CLL. (C) 2001 Elsevier Science Inc. All rights reserved.en_US
dc.format.extent8 - 13en_US
dc.titleComparative genomic hybridization and amplotyping by arbitrarily primed PCR in stage A B-CLLen_US
dc.typeJournal Article
rioxxterms.versionofrecord10.1016/S0165-4608(01)00470-8en_US
rioxxterms.licenseref.startdate2001-10-01en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfCANCER GENETICS AND CYTOGENETICSen_US
pubs.notesresearcherid-numbers: Calasanz, MJ/R-5813-2016 orcid-numbers: Calasanz, MJ/0000-0002-0374-3008 Martin-Subero, Jose Ignacio/0000-0001-8809-5195 Soto, Jose Luis/0000-0003-0234-9188 Zudaire, Maria Isabel/0000-0003-2297-7106 unique-id: ISI:000171742000002en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Clinical Studies
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Clinical Studies/Molecular Haematology (including Cytogenetics Group and Cell Markers)
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Molecular Haematology (including Cytogenetics Group and Cell Markers)
pubs.volume130en_US
pubs.embargo.termsNot knownen_US
icr.researchteamMolecular Haematology (including Cytogenetics Group and Cell Markers)en_US
dc.contributor.icrauthorMatutes, Estellaen_US


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