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dc.date.accessioned2018-09-25T10:33:37Z
dc.date.issued2001-05en_US
dc.identifierhttp://www.haematologica.org/cgi/reprint/86/5/464en_US
dc.identifier.citationHAEMATOLOGICA-THE HEMATOLOGY JOURNAL, 2001, 86 (5), pp. 464 - 469en_US
dc.identifier.issn0390-6078en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2862
dc.description.abstractPolycythemia vera: analysis of DNA from blood granulocytes using comparative genomic hybridization. Background and Objectives. The diagnosis of polycythemia vera (PV) is supported by the finding of an abnormal karyotype in patients with erythrocytosis. However, most W patients have normal marrow cytogenetics at presentation and there is reluctance to use this test routinely. Comparative genomic hybridization (CGH) is a cytogenetic screening technique that analyzes interphase cells. This approach offers practical advantages over conventional cytogenetics and interphase fluorescence insitu hybridization (IFISH), We have therefore evaluated the diagnostic utility of CGH applied to blood granulocytes in W. Design and Methods. Blood granulocytes from 17 PV patients were analyzed using CGH and the results compared with those from previous conventional cytogenetics and IFISH studies. Results. Three patients had abnormal CGH profiles. One case had gain of 9p. This patient had normal IFISH results using a centromere-g probe. The second case had complete gain of chromosomes 8 and 9 and the third had complete gain of chromosome 9, all confirmed by IFISH. Cytogenetics had not been performed in two of these cases and had failed in the third. Three cases with 20q deletion according to cytogenetics and/or IFISH, were normal by CGH. The remaining subjects were normal by all methods, Interpretation and Conclusions. CGH analysis of blood granulocytes can detect the chromosome gains commonly observed in PV. However, CGH cannot be relied on to detect 20q deletions, which are the most frequent cytogenetic abnormality in W. Thus, CGH has a role in the diagnosis and follow-up of W patients, but must be used in conjunction with other methods.en_US
dc.format.extent464 - 469en_US
dc.subjectPolycythemia vera; comparative genomic hybridization MYELOPROLIFERATIVE DISORDERS; MYELODYSPLASTIC SYNDROMES; 20Q DELETIONS; CHROMOSOME; MALIGNANCIES; CYTOGENETICS; PATHOGENESIS; TRISOMY-9; DIAGNOSIS; FISHen_US
dc.titlePolycythemia vera: analysis of DNA from blood granulocytes using comparative genomic hybridizationen_US
dc.typeJournal Article
rioxxterms.licenseref.startdate2001-05en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfHAEMATOLOGICA-THE HEMATOLOGY JOURNALen_US
pubs.issue5en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.volume86en_US
pubs.embargo.termsNot knownen_US
dc.contributor.icrauthorGruszka-Westwood, Alicjaen_US


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