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Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I

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Date
2001-04
ICR Author
Olson, Michael
Author
Coleman, ML
Sahai, EA
Yeo, M
Bosch, M
Dewar, A
Olson, MF
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Type
Journal Article
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Abstract
The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.
URI
https://repository.icr.ac.uk/handle/internal/2876
DOI
https://doi.org/10.1038/35070009
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  • Other ICR Research
Language
eng
License start date
2001-04
Citation
NATURE CELL BIOLOGY, 2001, 3 pp. 339 - 345
Publisher
MACMILLAN PUBLISHERS LTD

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