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dc.contributor.authorColeman, ML
dc.contributor.authorSahai, EA
dc.contributor.authorYeo, M
dc.contributor.authorBosch, M
dc.contributor.authorDewar, A
dc.contributor.authorOlson, MF
dc.date.accessioned2018-09-26T10:25:15Z
dc.date.issued2001-04
dc.identifier4
dc.identifier.citationNATURE CELL BIOLOGY, 2001, 3 pp. 339 - 345
dc.identifier.issn1465-7392
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2876
dc.identifier.doi10.1038/35070009
dc.description.abstractThe execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.
dc.format.extent339 - 345
dc.languageeng
dc.language.isoeng
dc.publisherMACMILLAN PUBLISHERS LTD
dc.titleMembrane blebbing during apoptosis results from caspase-mediated activation of ROCK I
dc.typeJournal Article
rioxxterms.versionofrecord10.1038/35070009
rioxxterms.licenseref.startdate2001-04
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNATURE CELL BIOLOGY
pubs.notesaffiliation: Coleman, ML (Reprint Author), Inst Canc Res, Chester Beatty Labs, CRC, Ctr Cellular & Mol Biol, 237 Fulham Rd, London SW3 6JB, England. Inst Canc Res, Chester Beatty Labs, CRC, Ctr Cellular & Mol Biol, London SW3 6JB, England. Royal Brompton Hosp, Natl Heart & Lung Inst, London SW3 6NP, England. keywords-plus: RHO-ASSOCIATED KINASE; BINDING PROTEIN-RHO; LIGHT-CHAIN PHOSPHORYLATION; ACTIN CYTOSKELETON; MYOSIN PHOSPHATASE; FOCAL ADHESIONS; PUTATIVE TARGET; REORGANIZATION; CELLS; PAK2 research-areas: Cell Biology web-of-science-categories: Cell Biology researcherid-numbers: Coleman, Mathew/C-1676-2014 Olson, Michael/A-3240-2011 orcid-numbers: Coleman, Mathew/0000-0001-6020-9023 Olson, Michael/0000-0003-3428-3507 Sahai, Erik/0000-0002-3932-5086 Bosch, Marta/0000-0003-3934-6781 number-of-cited-references: 32 times-cited: 722 usage-count-last-180-days: 2 usage-count-since-2013: 45 journal-iso: Nat. Cell Biol. doc-delivery-number: 421CE unique-id: ISI:000168043900011 da: 2018-09-26
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.volume3
pubs.embargo.termsNot known
dc.contributor.icrauthorOlson, Michaelen


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