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Design, synthesis, and evaluation of a novel pyrrolobenzodiazepine DNA-interactive agent with highly efficient cross-linking ability and potent cytotoxicity

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Date
2001-03-01
ICR Author
Kelland, Lloyd
Author
Gregson, SJ
Howard, PW
Hartley, JA
Brooks, NA
Adams, LJ
Jenkins, TC
Kelland, LR
Thurston, DE
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Type
Journal Article
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Abstract
A novel sequence-selective pyrrolobenzodiazepine (PBD) dimer 5 (SJG-136) has been developed that comprises two C2-exo-methylene-substituted DC-81 (3) subunits tethered through their C8 positions via an inert propanedioxy linker. This symmetric molecule is a highly efficient minor groove interstrand DNA cross-linking agent (XL(50) = 0.045 muM) that is 440-fold more potent than melphalan, Thermal denaturation studies show that, after 18 h incubation with calf thymus DNA at a 5:1 DNA/ligand ratio, it increases the T(m) value by 33.6 degreesC, the highest value so far recorded in this assay. The analogous dimer 4 (DSB-120) that lacks substitution/ unsaturation at the C2 position elevates melting by only 15.1 degreesC under the same conditions, illustrating the effect of introducing C2-exo-unsaturation which serves to flatten the C-rings and achieve a superior isohelical fit within the DNA minor groove. This behavior is supported by molecular modeling studies which indicate that (i) the PBD units are covalently bonded to guanines on opposite strands to form a cross-link, (ii) 5 has a greater binding energy compared to 4, and (iii) 4 and 5 have equivalent binding sites that span six base pairs. Dimer 5 is significantly more cytotoxic than 4 in a number of human ovarian cancer cell lines (e.g., IC(50) values of 0.0225 nM vs 7.2 nM, respectively, in A2780 cells). Furthermore, it retains full potency in the cisplatin-resistant cell line A2780cisR (0.024 nM), whereas 4 loses activity (0.21 muM) with a resistance factor of 29.2, This may be due to a lower level of inactivation of 5 by intracellular thiol-containing molecules. A dilactam analogue (21) of 5 that lacks the electrophilic N10-C11/N10’-C11’ imine moieties has also been synthesized and evaluated. Although unable to interact covalently with DNA, 21 still stabilizes the helix (DeltaT(m) = 0.78 degreesC) and has significant cytotoxicity in some cell lines (i.e., IC(50) = 0.57 muM in CH1 cells), presumably exerting its effect through noncovalent interaction with DNA.
URI
https://repository.icr.ac.uk/handle/internal/2885
DOI
https://doi.org/10.1021/jm001064n
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Language
eng
License start date
2001-03-01
Citation
JOURNAL OF MEDICINAL CHEMISTRY, 2001, 44 pp. 737 - 748

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