dc.contributor.author | Denis-Bacelar, AM | |
dc.contributor.author | Cronin, SE | |
dc.contributor.author | Da Pieve, C | |
dc.contributor.author | Paul, RL | |
dc.contributor.author | Eccles, SA | |
dc.contributor.author | Spinks, TJ | |
dc.contributor.author | Box, C | |
dc.contributor.author | Hall, A | |
dc.contributor.author | Sosabowski, JK | |
dc.contributor.author | Kramer-Marek, G | |
dc.contributor.author | Flux, GD | |
dc.date.accessioned | 2016-12-12T14:03:43Z | |
dc.date.issued | 2016-11-25 | |
dc.identifier.citation | EJNMMI research, 2016, 6 (1), pp. 85 - ? | |
dc.identifier.issn | 2191-219X | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/328 | |
dc.identifier.eissn | 2191-219X | |
dc.identifier.doi | 10.1186/s13550-016-0238-z | |
dc.description.abstract | BACKGROUND: Accurate quantification in molecular imaging is essential to improve the assessment of novel drugs and compare the radiobiological effects of therapeutic agents prior to in-human studies. The aim of this study was to investigate the challenges and feasibility of pre-clinical quantitative imaging and mouse-specific dosimetry of 111In-labelled radiotracers. Attenuation, scatter and partial volume effects were studied using phantom experiments, and an activity calibration curve was obtained for varying sphere sizes. Six SK-OV-3-tumour bearing mice were injected with 111In-labelled HER2-targeting monoclonal antibodies (mAbs) (range 5.58-8.52 MBq). Sequential SPECT imaging up to 197 h post-injection was performed using the Albira SPECT/PET/CT pre-clinical scanner. Mice were culled for quantitative analysis of biodistribution studies. The tumour activity, mass and percentage of injected activity per gram of tissue (%IA/g) were calculated at the final scan time point and compared to the values determined from the biodistribution data. Delivered 111In-labelled mAbs tumour absorbed doses were calculated using mouse-specific convolution dosimetry, and absorbed doses for 90Y-labelled mAbs were extrapolated under the assumptions of equivalent injected activities, biological half-lives and uptake distributions as for 111In. RESULTS: For the sphere sizes investigated (volume 0.03-1.17 ml), the calibration factor varied by a factor of 3.7, whilst for the range of tumour masses in the mice (41-232 mg), the calibration factor changed by a factor of 2.5. Comparisons between the mice imaging and the biodistribution results showed a statistically significant correlation for the tumour activity (r = 0.999, P < 0.0001) and the tumour mass calculations (r = 0.977, P = 0.0008), whilst no correlation was found for the %IA/g (r = 0.521, P = 0.29). Median tumour-absorbed doses per injected activity of 52 cGy/MBq (range 36-69 cGy/MBq) and 649 cGy/MBq (range 441-950 cGy/MBq) were delivered by 111In-labelled mAbs and extrapolated for 90Y-labelled mAbs, respectively. CONCLUSIONS: This study demonstrates the need for multidisciplinary efforts to standardise imaging and dosimetry protocols in pre-clinical imaging. Accurate image quantification can improve the calculation of the activity, %IA/g and absorbed dose. Diagnostic imaging could be used to estimate the injected activities required for therapeutic studies, potentially reducing the number of animals used. | |
dc.format | Print-Electronic | |
dc.format.extent | 85 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | SPRINGER HEIDELBERG | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.title | Pre-clinical quantitative imaging and mouse-specific dosimetry for 111In-labelled radiotracers. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2016-11-14 | |
rioxxterms.versionofrecord | 10.1186/s13550-016-0238-z | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2016-12 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | EJNMMI research | |
pubs.issue | 1 | |
pubs.notes | No embargo | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Preclinical Molecular Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Preclinical Molecular Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Radioisotope Physics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Radioisotope Physics/Radioisotope Physics (hon.) | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Radiotherapy Physics Modelling | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Preclinical Molecular Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Preclinical Molecular Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Radioisotope Physics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Radioisotope Physics/Radioisotope Physics (hon.) | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Radiotherapy Physics Modelling | |
pubs.publication-status | Published | |
pubs.volume | 6 | |
pubs.embargo.terms | No embargo | |
icr.researchteam | Preclinical Molecular Imaging | |
icr.researchteam | Radioisotope Physics | |
icr.researchteam | Radiotherapy Physics Modelling | |
dc.contributor.icrauthor | Denis-Bacelar, Ana | |
dc.contributor.icrauthor | Da Pieve, Chiara | |
dc.contributor.icrauthor | Box, Carol | |
dc.contributor.icrauthor | Kramer-Marek, Gabriela | |