Faecal neutrophil elastase-antiprotease balance reflects colitis severity.
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Date
2020-03-01ICR Author
Author
Barry, R
Ruano-Gallego, D
Radhakrishnan, ST
Lovell, S
Yu, L
Kotik, O
Glegola-Madejska, I
Tate, EW
Choudhary, JS
Williams, HRT
Frankel, G
Type
Journal Article
Metadata
Show full item recordAbstract
Given the global burden of diarrheal diseases on healthcare it is surprising how little is known about the drivers of disease severity. Colitis caused by infection and inflammatory bowel disease (IBD) is characterised by neutrophil infiltration into the intestinal mucosa and yet our understanding of neutrophil responses during colitis is incomplete. Using infectious (Citrobacter rodentium) and chemical (dextran sulphate sodium; DSS) murine colitis models, as well as human IBD samples, we find that faecal neutrophil elastase (NE) activity reflects disease severity. During C. rodentium infection intestinal epithelial cells secrete the serine protease inhibitor SerpinA3N to inhibit and mitigate tissue damage caused by extracellular NE. Mice suffering from severe infection produce insufficient SerpinA3N to control excessive NE activity. This activity contributes to colitis severity as infection of these mice with a recombinant C. rodentium strain producing and secreting SerpinA3N reduces tissue damage. Thus, uncontrolled luminal NE activity is involved in severe colitis. Taken together, our findings suggest that NE activity could be a useful faecal biomarker for assessing disease severity as well as therapeutic target for both infectious and chronic inflammatory colitis.
Collections
Subject
Neutrophils
Feces
Animals
Humans
Mice
Citrobacter rodentium
Enterobacteriaceae Infections
Colitis
Inflammatory Bowel Diseases
Disease Models, Animal
Disease Progression
Leukocyte Elastase
Dextran Sulfate
Acute-Phase Proteins
Serpins
Protease Inhibitors
Severity of Illness Index
Biomarkers
Language
eng
Date accepted
2019-11-07
License start date
2020-03
Citation
Mucosal immunology, 2020, 13 (2), pp. 322 - 333
Publisher
NATURE PUBLISHING GROUP